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MABF96 Anti-TRAcP Antibody, clone 9C5

Anti-TRAcP Antibody, clone 9C5 detects level of TRAcP & has been published & validated for use in IH, IP, DB, ELISA, IC & WB.

Anti-TRAcP Antibody, clone 9C5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Inflammation & Autoimmune Mechanisms Gene Symbol: ACP5 UniProt Number: P13686
MABF96
100 µg  
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Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIHC, IP, DB, ELISA, ICC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF96
DescriptionAnti-TRAcP Antibody, clone 9C5
Alternate Names
  • Tartrate-resistant acid phosphatase type 5
  • TR-AP
  • Tartrate-resistant acid ATPase
  • TrATPase
  • Type 5 acid phosphatase
Background InformationTartrate-resistant acid phosphatase type 5 (TRAcP), also referred to as Acp5, is secreted by osteoclasts during bone resorption. Release of TRAcP was stimulated by resorbogenic cytokines and repressed by calcitonin (a resorption inhibitor). TRAcP is also known to be expressed in high quantities in active macrophages and dendritic cells and play an essential part in presenting antigens to T cells. Inactive TRAcP released from adipose tissue macrophages has been shown to bring on hyperplastic obesity, keeping lipid metabolism and insulin sensitivity normal.
References
Product Information
FormatPurified
Control
  • Large malignant B-cells in hairy cell leukemia tissue
PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-TRAcP Antibody, clone 9C5 detects level of TRAcP & has been published & validated for use in IH, IP, DB, ELISA, IC & WB.
Key Applications
  • Immunohistochemistry
  • Immunoprecipitation
  • Dot Blot
  • ELISA
  • Immunocytochemistry
  • Western Blotting
Application NotesWestern Blot Analysis: A representative lot was used by an independent laboratory in WB. (Janckila, A.J., et al. (1995). Blood. 85:2839-2844.)

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Janckila, A.J., et al. (1995). Blood. 85:2839-2844.)

Dot Blot Analysis: A representative lot was used by an independent laboratory in DB. (Janckila, A.J., et al. (1995). Blood. 85:2839-2844.)

ELISA Analysis: A representative lot was used by an independent laboratory in ELISA. (Janckila, A.J., et al. (1995). Blood. 85:2839-2844.)

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. (Janckila, A.J., et al. (1996). The Journal of Histochemistry and Cytochemistry. 44(3):233-244.)
Biological Information
ImmunogenFull length purified protein corresponding to human TRAcP.
Clone9C5
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG2bκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq].
Gene Symbol
  • ACP5
Purification MethodProtein G
UniProt Number
UniProt SummaryFUNCTION: Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.

CATALYTIC ACTIVITY: A phosphate monoester + H2O = an alcohol + phosphate.

COFACTOR: Binds 2 iron ions per subunit.

SUBUNIT STRUCTURE: Exists either as monomer or, after proteolytic processing, as a dimer of two chains linked by disulfide bond(s).

SUBCELLULAR LOCATION: Lysosome.

SEQUENCE SIMILARITIES: Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.
Molecular Weight36 kDa calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in large malignant B-cells in hairy cell leukemia tissue.

Immunohistochemistry Analysis: 1:500 dilution of this antibody detected TRAcP in large malignant B-cells in hairy cell leukemia tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
MABF96 04053252750748

Documentation

Anti-TRAcP Antibody, clone 9C5 MSDS

Title

Safety Data Sheet (SDS) 

Anti-TRAcP Antibody, clone 9C5 Certificates of Analysis

TitleLot Number
Anti-TRAcP, clone 9C5 3068473
Anti-TRAcP, clone 9C5 - 2388986 2388986
Anti-TRAcP, clone 9C5 - 2464632 2464632
Anti-TRAcP, clone 9C5 - 2465454 2465454
Anti-TRAcP, clone 9C5 - 2055301 2055301
Anti-TRAcP, clone 9C5 - 2179372 2179372
Anti-TRAcP, clone 9C5 - 2283572 2283572
Anti-TRAcP, clone 9C5 - 2328390 2328390
Anti-TRAcP, clone 9C5 - 2367818 2367818
Anti-TRAcP, clone 9C5 - 3675947 3675947
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