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06-1376 Anti-SLBP Antibody

Use Anti-SLBP Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect SLBP also known as Histone stem-loop-binding protein, HBP, Histone RNA hairpin-binding protein.

Anti-SLBP Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins, Histones Gene Symbol: SLBP UniProt Number: P97440
06-1376
100 µL  
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Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, ICCRbSerumPolyclonal Antibody
Description
Catalogue Number06-1376
DescriptionAnti-SLBP Antibody
Alternate Names
  • Histone stem-loop-binding protein
  • hairpin binding protein
  • histone binding protein 2
  • histone stem-loop binding protein
  • stem-loop (histone) binding protein
  • stem-loop binding protein
  • HBP
  • Histone RNA hairpin-binding protein
Background InformationThe SLPB gene (stem-loop binding protein) encodes a protein that binds to the stem-loop structure of replication-dependent histone pre-mRNAs. Histone mRNA lack a polyadenylation signal. Consequently, the interaction of this protein with histone pre-mRNA contributes to efficient 3' end processing by stabilizing the complex between the pre-mRNA and U7 small nuclear ribonucleoprotein (snRNP). Expression of this protein is regulated during the cell cycle, increasing more than 10-fold during the latter part of G1.
References
Product Information
FormatSerum
Control
  • C2C12 cell lysates.
PresentationSerum in buffer containing 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationUse Anti-SLBP Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect SLBP also known as Histone stem-loop-binding protein, HBP, Histone RNA hairpin-binding protein.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry:
Representative lot data.
Confocal fluorescent analysis of NIH/3T3 cells using 06-1376 (Red). Actin filaments have been labeled with AlexaFluor®488 -Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains cytoplasm and nucleus.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human SLBP.
EpitopeC-terminus
HostRabbit
SpecificityAntibody recognizes SLBP.
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteReacts with Human and Mouse. Predicted to react with Rat based on sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a protein that binds to the stem-loop structure in replication-dependent histone mRNAs. Histone mRNAs do not contain introns or polyadenylation signals, and are processed by endonucleolytic cleavage. The stem-loop structure is essential for efficient processing but this structure also controls the transport, translation and stability of histone mRNAs. Expression of the protein is regulated during the cell cycle, increasing more than 10-fold during the latter part of G1. [provided by RefSeq]
Gene Symbol
  • SLBP
Purification MethodUnpurified
UniProt Number
UniProt SummaryFUNCTION:Binds the stem-loop structure of replication-dependent histone pre-mRNAs and contributes to efficient 3' end processing by stabilizing the complex between histone pre-mRNA and U7 small nuclear ribonucleoprotein (snRNP). Could play an important role in targeting mature histone mRNA from the nucleus to the cytoplasm and to the translation machinery. Stabilizes mature histone mRNA and could be involved in cell-cycle regulation of histone gene expression By similarity.
SUBUNIT STRUCTURE:: Monomer. SLBP/pre-mRNA complex interacts with ZNF473. Interacts with MIF4GD By similarity.
SUBCELLULAR LOCATION:: Cytoplasm. Nucleus. Note: Polyribosome-associated.
TISSUE SPECIFICITY: Widely expressed.
SEQUENCE SIMILARITIES: Belongs to the SLBP family.
Molecular Weight38 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in C2C12 cell lysates.

Western Blotting Analysis: A 1:1000 dilution of this antibody detected SLBP in C2C12 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
06-1376 04053252583391

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