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ABE440 Anti-RAG-1 Antibody

Detect RAG-1 using this rabbit polyclonal antibody, Anti-RAG-1 Antibody validated for use in western blotting, ICC & IP.

Anti-RAG-1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: RAG1, RNF74 UniProt Number: P15918
ABE440
100 µg  
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Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICC, IPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE440
DescriptionAnti-RAG-1 Antibody
Alternate Names
  • V(D)J recombination-activating protein 1
  • RAG-1
  • RING finger protein 74
Background InformationV(D)J recombination-activating protein 1 (RAG-1) is also called RING finger protein 74. RAG-1 is a component of the RAG complex, which mediates the DNA cleavage phase during V(D)J recombination. As part of the RAG complex, RAG1 mediates the DNA-binding and catalyzes the DNA cleavage activities by introducing a double-strand breaks between the recombination signal sequences (RSS) and the adjacent coding segment. RAG-1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3 as well as polyubiquitination of KPNA1. RAG-1 is expressed in maturing lymphoid cells. RAG-1 defects can cause disease states such as: combined cellular and humoral immune defects with granulomas (CHIDG), severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive (T-B-NK+ SCID), Omenn syndrome (OS), and alpha/beta T-cell lymphopenia with gamma/delta T-cell expansion severe cytomegalovirus infection and autoimmunity (T-CMVA).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect RAG-1 using this rabbit polyclonal antibody, Anti-RAG-1 Antibody validated for use in western blotting, ICC & IP.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunoprecipitation
Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected RAG-1 immunopreciptated from 500 µg of HL-60 cell lysate. (Note: It is recommended to immunoprecipitate RAG-1 before performing WB).

Immunocytochemistry Analysis: 1:100 dilution from a representative lot detected RAG-1 in Jurkat cells.

DyLight® is a registered trademark of Thermo Fisher Scientific.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a region near the N-terminus of human RAG-1.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • RAG1
  • RNF74
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~120 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunoprecipitation in HL-60 cell lysate.

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated RAG-1 in 500 µg of HL-60 cell lysate. Immunopreciptated protein was then probed with 1 µg/mL of the same antibody in WB.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
ABE440 04053252918032