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MABE341 Anti-Puromycin Antibody, clone 17H1

Anti-Puromycin antibody, clone 17H1 detects puromycin incorporated into protein. Monoclonal antibodies to puromycin may also be used with standard immunochemical methods.

Anti-Puromycin Antibody, clone 17H1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins
MABE341
100 µL  
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Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, AWBRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE341
DescriptionAnti-Puromycin Antibody, clone 17H1
Background InformationPuromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3' end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
References
Product Information
FormatPurified
HS Code3002 15 90
Control
  • HEK293 cell lysates treated with Puromycin and Cyclohexamide, or with Puromycin only.
PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Puromycin antibody, clone 17H1 detects puromycin incorporated into protein. Monoclonal antibodies to puromycin may also be used with standard immunochemical methods.
Key Applications
  • Western Blotting
Biological Information
ImmunogenPuromycin from Streptomyces alboniger
Clone17H1
HostRat
IsotypeIgG2aκ
Species Reactivity
  • Human
  • All
Species Reactivity NoteDemonstrated to react with Human test sample, preincubated with Puromycin. Predicted to react with all species when test sample is incubated with Puromycin.
Antibody TypeMonoclonal Antibody
Purification MethodProtein G Purified
Molecular WeightPuromycin is incorporated in neosynthesized proteins. In the presense of Puromycin only, this antibody detects Puromycin-incorporated neosynthesized proteins at multiple molecular weights. However, a decrease in signal is observed in the co-presense of Cycloheximide, an inhibitor of protein biosynthesis in eukaryotic organisms.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysates treated with Puromycin and Cyclohexamide, or with Puromycin only.

Western Blotting Analysis: A 1:100,000 dilution of this antibody detected Puromycin-incorporated neosynthesized proteins in HEK293 cell lysates treated with Puromycin only. This antibody also detected small mounts of Puromycin-incorporated neosynthesized proteins in HEK293 cells treated with Puromycin and Cyclohexamide.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
MABE341 04053252909214

Documentation

Anti-Puromycin Antibody, clone 17H1 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Puromycin Antibody, clone 17H1 Certificates of Analysis

TitleLot Number
Anti-Puromycin, clone 17H1 3082497
Anti-Puromycin, clone 17H1 - 3194622 3194622
Anti-Puromycin, clone 17H1 - 3258755 3258755
Anti-Puromycin, clone 17H1 - 3403489 3403489
Anti-Puromycin, clone 17H1 - 3600708 3600708
Anti-Puromycin, clone 17H1 - 3808797 3808797
Anti-Puromycin, clone 17H1 - 3928038 3928038
Anti-Puromycin, clone 17H1 - 3948852 3948852
Anti-Puromycin, clone 17H1 - 4093161 4093161
Anti-Puromycin, clone 17H1 - Q2192701 Q2192701
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