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539229 Anti-Protein Disulfide Isomerase Rabbit pAb

This Anti-Protein Disulfide Isomerase Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry, IP, Paraffin Sections for the detection of Protein Disulfide Isomerase.

Anti-Protein Disulfide Isomerase Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-PDI

539229
Purchase on Sigma-Aldrich

Przegląd

Replacement Information

Tabela kluczowych gatunków

Species ReactivityHostAntibody Type
B, Ca, Gp, Ht, H, Mk, M, Porcine, R, Sh, XnRbPolyclonal Antibody

Products

Numer katalogowyOpakowanie Ilość/opak.
539229-50UL Ampulka plastikowa 50 ul
Description
OverviewRecognizes the ~55 kDa (apparent MW) protein disulfide isomerase protein.
Catalogue Number539229
Brand Family Calbiochem®
SynonymsAnti-PDI
Application Data
Detection of protein disulfide isomerase immunoblotting. Samples: Purified bovine PDI (lane 1) and whole cell lysate from HeLa cells (lane 2). Primary antibody: Anti-Protein Disulfide Isomerase Rabbit pAb (Cat. No. 539229) (1:1000). Detection: chemiluminescence.
References
ReferencesCribb, A.E., et al. 1997. J. Pharmacol. Exp. Ther. 282, 1064.
Freedman, R.B., and Hillson, D.A. 1980. In Enzymology of post-translational modification of proteins. (Freedman R.B. and Hawkin H.C. eds.) Academic, London p. 157.
Product Information
FormLiquid
FormulationIn PBS, 50% glycerol.
Positive controlHeLa cells, rat testis, mouse spleen
Preservative≥0.9% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Paraffin Sections
Application NotesImmunoblotting (1:1000, colorimetric)
Immunocytochemistry (see comments)
Immunoprecipitation (see comments)
Paraffin Sections (see comments)
Application CommentsThis antibody will also work in immunocytochemistry, immunoprecipitation, and paraffin sections; the concentration must be determined for each sample analyzed. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogenbovine liver Protein Disulfide Isomerase
ImmunogenBovine
HostRabbit
IsotypeIgG
Species Reactivity
  • Bovine
  • Canine
  • Guinea Pig
  • Hamster
  • Human
  • Monkey
  • Mouse
  • Porcine
  • Rat
  • Sheep
  • Xenopus
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
539229-50UL 04055977268973

Documentation

Anti-Protein Disulfide Isomerase Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

Anti-Protein Disulfide Isomerase Rabbit pAb Certificates of Analysis

TitleLot Number
539229

References

Przegląd literatury
Cribb, A.E., et al. 1997. J. Pharmacol. Exp. Ther. 282, 1064.
Freedman, R.B., and Hillson, D.A. 1980. In Enzymology of post-translational modification of proteins. (Freedman R.B. and Hawkin H.C. eds.) Academic, London p. 157.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-March-2012 JSW
SynonymsAnti-PDI
ApplicationImmunoblotting (1:1000, colorimetric)
Immunocytochemistry (see comments)
Immunoprecipitation (see comments)
Paraffin Sections (see comments)
Application Data
Detection of protein disulfide isomerase immunoblotting. Samples: Purified bovine PDI (lane 1) and whole cell lysate from HeLa cells (lane 2). Primary antibody: Anti-Protein Disulfide Isomerase Rabbit pAb (Cat. No. 539229) (1:1000). Detection: chemiluminescence.
DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~55 kDa (apparent MW) protein disulfide isomerase protein.
BackgroundPDI is an abundant component of the ER that catalyzes disulfide bond formation in the biosynthesis of various secretory and cell surface proteins. It is a multi-functional polypeptide identical to the β-subunit of prolyl 4-hydroxylase and a cellular thyroid hormone-binding protein. It has two thioredoxin-like regions, each con-taining a Cys-Gly-His-Cys sequence that may represent two independently acting catalytic sites of PDI activity. The C-terminus of PDI has a KDEL sequence, which acts as retention signal for proteins of the ER lumen. PDI exists as a homodimer of ~107 kDa. Prolyl-4-hydroxylase, an α2β2 tetramer, catalyzes the formation of 4-hydroxyproline in collagen by the hydroxylation of proline residues in peptide linkages. The PDI/β-subunit of prolyl-4-hydroxylase is required for its retention in the ER.
HostRabbit
Immunogen speciesBovine
Immunogenbovine liver Protein Disulfide Isomerase
IsotypeIgG
SpeciesXenopus, bovine, canine, guinea pig, hamster, human, monkey, mouse, porcine, rat, sheep
Positive controlHeLa cells, rat testis, mouse spleen
FormLiquid
FormulationIn PBS, 50% glycerol.
Preservative≥0.9% sodium azide
CommentsThis antibody will also work in immunocytochemistry, immunoprecipitation, and paraffin sections; the concentration must be determined for each sample analyzed. Variables associated with assay conditions will dictate the optimal working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesCribb, A.E., et al. 1997. J. Pharmacol. Exp. Ther. 282, 1064.
Freedman, R.B., and Hillson, D.A. 1980. In Enzymology of post-translational modification of proteins. (Freedman R.B. and Hawkin H.C. eds.) Academic, London p. 157.