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MAB5240 Anti-Phosphacan Antibody

Detect Phosphacan using this Anti-Phosphacan Antibody validated for use in ELISA, WB, IH(P).

Anti-Phosphacan Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Growth Cones & Axon Guidance Gene Symbol: PTPRZ1, PTPRZ, PTPZ UniProt Number: Q62656
MAB5240
1 mL  
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Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
ChELISA, WB, IH(P)MCulture SupernatantMonoclonal Antibody
Description
Catalogue NumberMAB5240
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Phosphacan Antibody
Background InformationPhosphacan is a soluble nervous tissue-specific proteoglycan that is thought to regulate axonal outgrowth. It is synthesized by glia and binds to neurons and to the neural cell adhesion molecules tenascin, N-CAM or NG-CAM but not to laminin and fibronectin. Phosphacan acts as a potent inhibitor of cell adhesion and neurite outgrowth.
References
Product Information
FormatCulture Supernatant
PresentationTissue culture supernatant. Liquid. Contains no preservative.
Quality LevelMQ100
Applications
ApplicationDetect Phosphacan using this Anti-Phosphacan Antibody validated for use in ELISA, WB, IH(P).
Key Applications
  • ELISA
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesWestern blot: 1:1,000

Samples must be digested with chondroitinase prior to running on SDS gels because undigested phosphacan is too large for most gels. Treatment is at a concentration of chondroitinase of 10U/mL in Tris-HCL pH 8.0. Make tissue or cell extract in 20-50mM Tris pH 7.6-8.0 with 0.15M NaCl in the presence of protease inhibitors. Add 1 microliter of enzyme to 30 microliters of extract and incubate 30 minutes at 37C. Then add SDS sample buffer, heat or boil sample as normal for SDS reducing samples.

Immunohistochemistry: 1:20 Works on cold methanol or paraformaldehyde fixed tissues. Works on paraffin embedded tissues. Chondrotinase treatment of tissue consists of treating mounted tissue section with a solution of PBS plus 0.5U/mL of Chondroitinase ABC for 30 minutes at room temperature prior to blocking and antibody staining.

Immunocytochemistry: not tested

ELISA: Excellent for core and monomer protein.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenMixture of chicken chondroitin sulfate proteoglycans.
HostMouse
SpecificityPhosphacan (Receptor Tyrosine Phosphatase Beta).
IsotypeIgM
Species Reactivity
  • Chicken
Antibody TypeMonoclonal Antibody
Gene Symbol
  • PTPRZ1
  • PTPRZ
  • PTPZ
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size1 mL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
MAB5240 04053252398704

Documentation

Anti-Phosphacan Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-Phosphacan Antibody Certificates of Analysis

TitleLot Number
MOUSE ANTI-PHOSPHACAN (CHICKEN) (RECEPTOR TYROSINE PHOSPHATASE BETA) MONOCLONAL ANTIBODY - 2341911 2341911
MOUSE ANTI-PHOSPHACAN (CHICKEN) (RECEPTOR TYROSINE PHOSPHATASE BETA) - 3387590 3387590
MOUSE ANTI-PHOSPHACAN (CHICKEN) (RECEPTOR TYROSINE PHOSPHATASE BETA) - 3761083 3761083
MOUSE ANTI-PHOSPHACAN (CHICKEN) (RECEPTOR TYROSINE PHOSPHATASE BETA) - 4008322 4008322
MOUSE ANTI-PHOSPHACAN (CHICKEN) (RECEPTOR TYROSINE PHOSPHATASE BETA) MONOCLONAL ANTIBODY 3150379
MOUSE ANTI-PHOSPHACAN (CHICKEN) (RECEPTOR TYROSINE PHOSPHATASE BETA) MONOCLONAL ANTIBODY 3114930
MOUSE ANTI-PHOSPHACAN (CHICKEN) - 3991560 3991560
MOUSE ANTI-PHOSPHACAN (CHICKEN) - 4159434 4159434
MOUSE ANTI-PHOSPHACAN (CHICKEN) -2622640 2622640
MOUSE ANTI-PHOSPHACAN (CHICKEN) -2657453 2657453
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