Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

CP35 Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8)

This Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of PSD-95 (Ab-2).

Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
CP35
Purchase on Sigma-Aldrich

Przegląd

Replacement Information

Tabela kluczowych gatunków

Species ReactivityHostAntibody Type
RMMonoclonal Antibody

Products

Numer katalogowyOpakowanie Ilość/opak.
CP35-100UL Butelka szklana 100 ul
Description
OverviewRecognizes the ~95 kDa PSD95 protein.
Catalogue NumberCP35
Brand Family Calbiochem®
Application Data
Detection of rat PSD-95 by immunofluorescence. Sample: Rat hippocampal cells. Primary antibody: Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8) (Cat. No. CP35) (1:200). Detection: fluorescence.
References
ReferencesBrakeman, P.R., et al. 1997. Nature 386, 284.
Hualing, D., et al. 1997. Nature 386, 279.
Kennedy, M.B. 1997. Trends in Neurosci. 20, 264.
Sanes, J. 1997. Curr. Opin. Neurobiol. 7, 93.
Cho., K.-O., et al. 1992. Neuron 9, 929.
Product Information
FormLiquid
FormulationIn PBS.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesOriginal Clone Kornau, H.-C., et al. 1995. Science 269, 1737.
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Application NotesImmunoblotting (1:2000)
Immunofluorescence (1:200, indirect)
Immunoprecipitation (see comments)
Application CommentsImmunofluorescent staining of rat hippocampal cells shows a staining pattern coincident with NMDA receptor staining at synaptic sites consistent with PSD-95's proposed role in receptor clustering. This antibody has also been reported to work for immunoprecipitation. Fixation with cold methanol is recommended for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, rat PSD95
ImmunogenRat
Clone7E3-1B8
HostMouse
IsotypeIgG2a
Species Reactivity
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
CP35-100UL 04055977220988

Documentation

Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8) MSDS

Title

Safety Data Sheet (SDS) 

Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8) Certificates of Analysis

TitleLot Number
CP35

References

Przegląd literatury
Brakeman, P.R., et al. 1997. Nature 386, 284.
Hualing, D., et al. 1997. Nature 386, 279.
Kennedy, M.B. 1997. Trends in Neurosci. 20, 264.
Sanes, J. 1997. Curr. Opin. Neurobiol. 7, 93.
Cho., K.-O., et al. 1992. Neuron 9, 929.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision21-August-2007 RFH
ApplicationImmunoblotting (1:2000)
Immunofluorescence (1:200, indirect)
Immunoprecipitation (see comments)
Application Data
Detection of rat PSD-95 by immunofluorescence. Sample: Rat hippocampal cells. Primary antibody: Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8) (Cat. No. CP35) (1:200). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with HL-1 mouse myeloma cells (see application references). Recognizes the ~95 kDa PSD-95 protein.
BackgroundEffective synaptic transmission requires the precise distribution and spatial localization of neurotransmitter receptors and ion channels on the postsynaptic nerve terminal. An understanding of synaptic development is derived largely from studies of the vertebrate skeletal neuromuscular junction. At glutamatergic synapses, the membrane associated cytoskeletal protein PSD-95 (Post Synaptic Density 95kDa) is believed to promote receptor clustering via interactions with the NR2 subunit of the NMDA receptor and the Shaker-type potassium channel. These interactions are likely mediated by multiple repeating PDZ domains (90 amino acid repeats). Two additional proteins, homer and GRIP, have recently been shown to interact with the metabotropic and AMPA receptors, respectively. It is likely that these proteins serve major functions in the molecular scaffolding and signal transduction process, which occurs at the synaptic junction.
HostMouse
Immunogen speciesRat
Immunogenrecombinant, rat PSD95
Clone7E3-1B8
IsotypeIgG2a
Speciesrat
FormLiquid
FormulationIn PBS.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsImmunofluorescent staining of rat hippocampal cells shows a staining pattern coincident with NMDA receptor staining at synaptic sites consistent with PSD-95's proposed role in receptor clustering. This antibody has also been reported to work for immunoprecipitation. Fixation with cold methanol is recommended for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesBrakeman, P.R., et al. 1997. Nature 386, 284.
Hualing, D., et al. 1997. Nature 386, 279.
Kennedy, M.B. 1997. Trends in Neurosci. 20, 264.
Sanes, J. 1997. Curr. Opin. Neurobiol. 7, 93.
Cho., K.-O., et al. 1992. Neuron 9, 929.
Application referencesOriginal Clone Kornau, H.-C., et al. 1995. Science 269, 1737.