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05-718 Anti-PML Antibody, clone 36.1-104

05-718
200 µg  
Purchase on Sigma-Aldrich

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Species ReactivityKey ApplicationsHostFormatAntibody Type
MICC, WBMPurifiedMonoclonal Antibody
Description
Catalogue Number05-718
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-PML Antibody, clone 36.1-104
Background InformationPromyeloctic Leukemia Gene product (PML) is a nuclear protein with growth-suppressive properties originally identified in the context of the PML-retinoic receptor alpha (RAR alpha) fusion protein of acute promyelocytic leukemia. PML localizes within distinct nuclear structures, called nuclear bodies, which are disrupted by the expression of PML-RAR alpha.
References
Product Information
FormatPurified
Control
  • MEF1 cell lysate
PresentationProtein G Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide as a preservative.
Quality LevelMQ100
Applications
ApplicationDetect PML also known as Promyelocytic Leukemia Protein with Anti-PML Antibody, clone 36.1-104 (Mouse Monoclonal Antibody), that has been demonstrated to work in ICC & WB.
Key Applications
  • Immunocytochemistry
  • Western Blotting
Biological Information
Immunogen6His fusion protein corresponding to mouse PML (promyelocytic leukemia protein)
Clone36.1-104
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityRecognizes PML.
IsotypeIgG
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.
Gene Symbol
  • PML
  • PP8675
  • RNF71
  • TRIM19
  • MYL
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P29590 # Probable transcription factor. May play an important role in recruitment of ELF4 into PML nuclear bodies.
SIZE: 882 amino acids; 97517 Da
SUBUNIT: Interacts with SIRT1, TOPBP1, TRIM27 and TRIM69. Interacts with the C-terminus of ELF4. Interacts with Lassa virus Z protein and rabies virus phosphoprotein (By similarity).
SUBCELLULAR LOCATION: Nucleus. Note=Localized to PML nuclear bodies.
PTM: Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
DISEASE: SwissProt: P29590 # A chromosomal aberration involving PML may be a cause of acute promyelocytic leukemia (APL). Translocation t(15;17)(q21;q21) with RARA. The PML breakpoints (type A and type B) lie on either side of an alternatively spliced exon.
SIMILARITY: SwissProt: P29590 ## Contains 2 B box-type zinc fingers. & Contains 1 RING-type zinc finger.
Molecular Weight90-106 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from mouse embryo fibroblast (MEF1) cells
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size200 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
05-718 04053252333743

Documentation

Anti-PML Antibody, clone 36.1-104 MSDS

Title

Safety Data Sheet (SDS) 

Anti-PML Antibody, clone 36.1-104 Certificates of Analysis

TitleLot Number
Anti-PML, clone 36.1-104 (mouse monoclonal IgG2b) - 2089011 2089011
Anti-PML, clone 36.1-104 (mouse monoclonal IgG2b) 2872330
Anti-PML, clone 36.1-104 (mouse monoclonal IgG2b) - 2052862 2052862
Anti-PML, clone 36.1-104 (mouse monoclonal IgG2b) - 2196189 2196189
Anti-PML, clone 36.1-104 - 24520 24520
Anti-PML, clone 36.1-104 - 2477976 2477976
Anti-PML, clone 36.1-104 - 3680799 3680799
Anti-PML, clone 36.1-104 - 3810799 3810799
Anti-PML, clone 36.1-104 - 3989079 3989079
Anti-PML, clone 36.1-104 - DAM1631993 DAM1631993

References

Reference overviewApplicationPub Med ID
Combining the differentiating effect of panobinostat with the apoptotic effect of arsenic trioxide leads to significant survival benefit in a model of t(8;21) acute myeloid leukemia.
Salmon, JM; Bots, M; Vidacs, E; Stanley, KL; Atadja, P; Zuber, J; Johnstone, RW
Clinical epigenetics  7  2  2015

Pokaż streszczenie
Western Blotting25628765 25628765
Senescence induced by RECQL4 dysfunction contributes to Rothmund-Thomson syndrome features in mice.
Lu, H; Fang, EF; Sykora, P; Kulikowicz, T; Zhang, Y; Becker, KG; Croteau, DL; Bohr, VA
Cell death & disease  5  e1226  2014

Pokaż streszczenie
Immunofluorescence24832598 24832598
SIRT1 stabilizes PML promoting its sumoylation.
Campagna, M; Herranz, D; Garcia, MA; Marcos-Villar, L; González-Santamaría, J; Gallego, P; Gutierrez, S; Collado, M; Serrano, M; Esteban, M; Rivas, C
Cell death and differentiation  18  72-9  2010

Pokaż streszczenie
Immunofluorescence20577263 20577263
Senescent mouse cells fail to overtly regulate the HIRA histone chaperone and do not form robust Senescence Associated Heterochromatin Foci.
Alyssa L Kennedy,Tony McBryan,Greg H Enders,F Brad Johnson,Rugang Zhang,Peter D Adams
Cell division  5  2009

Pokaż streszczenie Pełny tekst artykułu
20569479 20569479
Assembly dynamics of PML nuclear bodies in living cells.
Brand, P; Lenser, T; Hemmerich, P
PMC biophysics  3  3  2009

Pokaż streszczenie Pełny tekst artykułu
20205709 20205709
HDAC3 as a molecular chaperone for shuttling phosphorylated TR2 to PML: a novel deacetylase activity-independent function of HDAC3.
Gupta, P; Ho, PC; Ha, SG; Lin, YW; Wei, LN
PloS one  4  e4363  2009

Pokaż streszczenie Pełny tekst artykułu
19204783 19204783
ERG-associated protein with SET domain (ESET)-Oct4 interaction regulates pluripotency and represses the trophectoderm lineage.
Yeap, LS; Hayashi, K; Surani, MA
Epigenetics & chromatin  2  12  2009

Pokaż streszczenie
Western Blotting19811652 19811652
Retinoic acid-stimulated sequential phosphorylation, PML recruitment, and SUMOylation of nuclear receptor TR2 to suppress Oct4 expression.
Gupta, P; Ho, PC; Huq, MM; Ha, SG; Park, SW; Khan, AA; Tsai, NP; Wei, LN
Proceedings of the National Academy of Sciences of the United States of America  105  11424-9  2008

Pokaż streszczenie Pełny tekst artykułu
18682553 18682553
SUMO-1-dependent allosteric regulation of thymine DNA glycosylase alters subnuclear localization and CBP/p300 recruitment.
Mohan, RD; Rao, A; Gagliardi, J; Tini, M
Molecular and cellular biology  27  229-43  2007

Pokaż streszczenie
17060459 17060459
Direct transcriptional activation of promyelocytic leukemia protein by IFN regulatory factor 3 induces the p53-dependent growth inhibition of cancer cells.
Kim, TK; Lee, JS; Oh, SY; Jin, X; Choi, YJ; Lee, TH; Lee, Eh; Choi, YK; You, S; Chung, YG; Lee, JB; DePinho, RA; Chin, L; Kim, H
Cancer research  67  11133-40  2007

Pokaż streszczenie
Western Blotting18056437 18056437