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MABD20 Anti-PAX7 Antibody, clone 2F12H4

Anti-PAX7 Antibody, clone 2F12H4 is a Rat monoclonal antibody for detection of PAX7 also known as paired box homeotic gene 7, paired box protein Pax-7, paired domain gene 7 & has been validated in WB, ICC.

Anti-PAX7 Antibody, clone 2F12H4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Transcription Factors Gene Symbol: PAX7, HUP1, RMS2, PAX7B, Hup1, HuP1 UniProt Number: P47239
MABD20
100 µg  
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Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HWB, ICC, ChIPRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABD20
DescriptionAnti-PAX7 Antibody, clone 2F12H4
Alternate Names
  • paired box 7
  • PAX7 transcriptional factor
  • paired box gene 7
  • paired box homeotic gene 7
  • paired box protein Pax-7
  • paired domain gene 7
Background InformationPaired box protein Pax-7 (PAX7) belongs to the Pax family of transcription factors, a highly conserved group of proteins with crucial roles in tissue homeostasis and organogenesis. PAX7 is involved in the specification, survival, maintenance, and self-renewal of skeletal muscle progenitors and myogenic progenitors. It is primarily expressed in newly activated and quiescent satellite cells. Studies have shown that on activation, quiescent satellite cells co-express MyoD with PAX7 prior to proliferation, after which one or the other transcription factor is downregulated. Downregulation of MyoD instead of PAX7 leads to withdrawal from the cell cycle and myogenic differentiation, causing the cell to return to seemingly quiescent state. Chromosomal aberrations relating to PAX7 have been linked to rhabdomysarcoma 2 (RMS2).
References
Product Information
FormatPurified
HS Code3002 15 90
Control
  • Mouse fetal brain tissue lysate
PresentationPurified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-PAX7 Antibody, clone 2F12H4 is a Rat monoclonal antibody for detection of PAX7 also known as paired box homeotic gene 7, paired box protein Pax-7, paired domain gene 7 & has been validated in WB, ICC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Chromatin Immunoprecipitation (ChIP)
Application NotesImmunocytochemistry: A previous lot of this antibody was shown to detect Pax7 localized in euchromatin of human Ewing’s
sarcoma cells. The antibodies are detected with Alexa Fluor® 488-conjugated anti-rat IgG. Each sample is counterstained with hoechst (Harada, A., et al. (2009). Hybridoma, 26:6. Hyb.2009.0039).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to mouse PAX7.
Clone2F12H4
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRat
IsotypeIgG2bκ
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteDemonstrated to react with mouse. Shown to react with human by an independent laboratory (Harada, A., et al. (2009). Hybridoma, 26:6. Hyb.2009.0039).
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PAX7
  • HUP1
  • RMS2
  • PAX7B
  • Hup1
  • HuP1
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Probable transcription factor. May have a role in myogenesis.

SUBUNIT STRUCTURE: Can bind to DNA as a heterodimer with PAX3. Interacts with DAXX (By similarity).

SUBCELLULAR LOCATION: Nucleus.

DEVELOPMENTAL STAGE: Expressed specifically during the development of the nervous and muscular system.

SEQUENCE SIMILARITIES: Belongs to the paired homeobox family.

Contains 1 homeobox DNA-binding domain.

Contains 1 paired domain.
Molecular Weight ~ 55 kDa observed.
The calculated MW of PAX7 is 55 kDa; however, expression of PAX7 can have multiple isoforms. (Robert B., et al. (2008). Physiol. Genomics 33:41-49.)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in mouse fetal brain tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected PAX7 in 10 µg of mouse fetal brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
MABD20 04053252422508

Documentation

Anti-PAX7 Antibody, clone 2F12H4 MSDS

Title

Safety Data Sheet (SDS) 

Anti-PAX7 Antibody, clone 2F12H4 Certificates of Analysis

TitleLot Number
Anti-PAX7, clone 2F12H4 - 2366000 2366000
Anti-PAX7, clone 2F12H4 - 2449223 2449223
Anti-PAX7, clone 2F12H4 - 2452466 2452466
Anti-PAX7, clone 2F12H4 - 1990709 1990709
Anti-PAX7, clone 2F12H4 - 2565833 2565833
Anti-PAX7, clone 2F12H4 - 3472420 3472420
Anti-PAX7, clone 2F12H4 - 3979353 3979353
Anti-PAX7, clone 2F12H4 - 4163004 4163004
Anti-PAX7, clone 2F12H4 - NRG1803608 NRG1803608
Anti-PAX7, clone 2F12H4 -2673125 2673125
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