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MAB4092 Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1

Detect P-Selectin Glycoprotein Ligand-1 using this Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1 validated for use in FC, IH, IH(P), IP & WB.

Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Inflammation & Autoimmune Mechanisms Gene Symbol: SELPLG, CLA, PSGL-1, CD162, PSGL1 UniProt Number: Q14242
MAB4092
100 µg  
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Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, IHC, IH(P), IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB4092
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1
Alternate Names
  • PSGL-1
  • CD162
Background InformationInteractions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest “rolling” of leukocytes on the lumenal surface of endothelial cells at sites of inflammation. To date, the primary role of PSGL-1 in mediating the interaction between neutrophils and P-selectin has been well documented. MAB4092 completely blocks recognition of PSGL-1 by either P-selectin or by L-selectin, but does not affect leukocyte recognition of E-selectin. Two color flow cytometry of normal leukocytes has demonstrated that PSGL-1 is expressed on essentially all blood neutrophils, NK cells, blood cells, T cells, and monocytes, but PSGL-1 stains B cells at significantly lower levels than other cell types. Variation in tyrosine sulfation during B cell differentiation may affect the stability of B cells to interact with P- and L-selectin. {Snapp, KR et al 1998}.
References
Product Information
FormatPurified
PresentationPurified Immunoglobulin. Liquid in 0.02M PB with 0.25M NaCl, pH7.6, 0.1% Sodium Azide.
Quality LevelMQ100
Applications
ApplicationDetect P-Selectin Glycoprotein Ligand-1 using this Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1 validated for use in FC, IH, IH(P), IP & WB.
Key Applications
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern blot of HL60 whole cell lysates prepared with 1% triton X-100 in 150mM NaCl, 10mM Tris-HCL pH 7.6, 1 mM Ca++, 1 mM Mg++, with 1mmol aprotinin, PMSF,leupeptin, and pepstatin A {Snapp, KR et al 1998}. 1:1000

FACS {Snapp, KR et al 1998}.

Immunoprecipitation: 0.5% triton X-100 in TBS with proteinase inhibitors; 5 µg/500µL of whole cell lysate (350-500mg/mL total protein).

Functional blocking of P-selectin {Snapp KR et al 1998}.

Immunohistochemistry: Fresh frozen, acetone fixed sections

Immunohistochemistry in paraffin embedded tissues: formalin fixed, pretreatment standard citric acid pH 6.0, microwave treatment is recommended 1:50-1:200

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenThe antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin).
CloneKPL-1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityP-selectin glycoprotein ligand-1 (PSGL-1). The antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin). {Snapp KR et al. 1998}. The binding of KPL-1 to human PSGL-1 on HL-60 cells does not require sulfation {Thatte, A et al 2002}.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteKnown not to react with Rat, Bovine, Pig, and Equine PSGL-1 {Snapp, KR et al 1998; Baisse, B et al 2007}.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummarySELPLG is the high affinity counter-receptor for P-selectin on myeloid cells and stimulated T lymphocytes. As such, it plays a critical role in the tethering of these cells to activated platelets or endothelia expressing P-selectin. The organization of the SELPG gene closely resembles that of CD43 and the human platelet glycoprotein GpIb-alpha both of which have an intron in the 5-prime-noncoding region, a long second exon containing the complete coding region, and TATA-less promoters.
Gene Symbol
  • SELPLG
  • CLA
  • PSGL-1
  • CD162
  • PSGL1
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q14242 # Binds to P-, E- and L-selectins. The calcium-dependent high affinity interaction with P-selectin mediates the tethering and rolling of neutrophils and T-lymphocytes on endothelial cells.
SIZE: 412 amino acids; 43201 Da
SUBUNIT: Homodimer; disulfide-linked.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Expressed on neutrophils, monocytes and most lymphocytes.
PTM: Displays complex, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N- acetyllactosamine with varying degrees of substitution. Also N- glycosylated. & Sulfated in the N-terminal region; sulfation is necessary for P-selectin binding.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C in undiluted aliquots up to 12 months.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
MAB4092 04053252667763

Documentation

Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1 MSDS

Title

Safety Data Sheet (SDS) 

Anti-P-Selectin Glycoprotein Ligand-1 Antibody, clone KPL-1 Certificates of Analysis

TitleLot Number
MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] MONOCLONAL ANTIBODY - 2383971 2383971
MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] MONOCLONAL ANTIBODY - 2359959 2359959
MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] - 3231554 3231554
MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] - 3680716 3680716
MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] - 3946452 3946452
MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] - 3959180 3959180
MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] - 4135337 4135337
MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] -2594838 2594838
MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] -2614679 2614679
MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] -2635046 2635046
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References

Reference overviewApplicationPub Med ID
Kinetic measurements of cell surface E-selectin/carbohydrate ligand interactions.
Long, M, et al.
Annals of biomedical engineering, 29: 935-46 (2001)  2001

Pokaż streszczenie 		Inhibits Activity/Function11791676 11791676

Data Sheet

Title
MOUSE ANTI-P-SELECTIN GLYCOPROTEIN LIGAND-1 [PSGL-1] MONOCLONAL ANTIBODY