Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

06-284-AF647 Anti-Nitrotyrosine Antibody AlexaFluor® 647 Conjugate

Anti-Nitrotyrosine AlexaFluor® 647 Conjugate, Cat. No. 06-284-AF647, is a rabbit polyclonal antibody that detects tyrosine nitrated proteins and has been tested for use in Immunocytochemistry.

Anti-Nitrotyrosine Antibody AlexaFluor® 647 Conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling
06-284-AF647
100 µL  
Purchase on Sigma-Aldrich

Oferty specjalne

Przegląd

Replacement Information

Oferty specjalne

100% Performance Guaranteed

Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICCRbAlexaFluor®647Polyclonal Antibody
Description
Catalogue Number06-284-AF647
DescriptionAnti-Nitrotyrosine Antibody AlexaFluor® 647 Conjugate
Alternate Names
  • Nitrotyrosine AlexaFluor® 647 Conjugate
Background InformationNitrotyrosine is produced by the action of reactive nitrogen species, such as peroxinitrite and nitrogen dioxide on tyrosine residues in proteins. Nitrotyrosine is an indicator of cell damage, inflammation, and nitric oxide production. Both free tyrosine and protein bound tyrosine residues are prone to nitration.
In several pathological conditions, oxidative stress is increased resulting in the formation of super oxide and nitric oxide that react to form peroxynitrite (ONOO-), which is a highly reactive molecule. It causes oxidation of proteins and lipids and DNA damage, which results in loss of protein and lipid function and can increase the probability of gene mutations. Higher incidence of protein nitration has been reported in diabetes, atherosclerotic plaques, and rheumatoid arthritis, among others. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected cells and tissues by immunocytochemical techniques using antibodies developed against the nitrotyrosine.
References
Product Information
FormatAlexaFluor®647
PresentationPurified rabbit polyclonal antibody in buffer containing PBS, pH7.1, and 15 mg/mL BSA with 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Nitrotyrosine AlexaFluor® 647 Conjugate, Cat. No. 06-284-AF647, is a rabbit polyclonal antibody that detects tyrosine nitrated proteins and has been tested for use in Immunocytochemistry.
Key Applications
  • Immunocytochemistry
Application NotesThe unconjugated antibody (Cat. No. 06-284) is shown to be suitable also for Western blotting, immunocytochemistry, immunohistochemistry, and immunoprecipitation.
Biological Information
ImmunogenNitrated Keyhole Limpet hemocyanain (KLH)
EpitopeNitrated Tyrosine
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects tyrosine nitrated proteins.
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with all species.
Antibody TypePolyclonal Antibody
Purification MethodAffinity Purfied
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in A431 treated with peroxytnitrite.

Immunocytochemistry Analysis: 1:100 dilution of this antibody detected Nitrotyrosine in A431 cells treated with 5 mM peroxytnitrite.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
06-284-AF647 04055977298925

Documentation

Anti-Nitrotyrosine Antibody AlexaFluor® 647 Conjugate MSDS

Title

Safety Data Sheet (SDS) 

Anti-Nitrotyrosine Antibody AlexaFluor® 647 Conjugate Certificates of Analysis

TitleLot Number
Anti-Nitrotyrosine AlexaFluor® 647 -Q2610562 Q2610562
Anti-Nitrotyrosine AlexaFluor® 647 Conjugate 2872135
Anti-Nitrotyrosine AlexaFluor® 647 Conjugate - 3313715 3313715
Anti-Nitrotyrosine AlexaFluor® 647 Conjugate - 3442503 3442503
Anti-Nitrotyrosine AlexaFluor® 647 Conjugate - 3524750 3524750
Anti-Nitrotyrosine AlexaFluor® 647 Conjugate - 3995072 3995072
Anti-Nitrotyrosine AlexaFluor® 647 Conjugate - 4029337 4029337
Anti-Nitrotyrosine AlexaFluor® 647 Conjugate Polyclonal Antibody 3053797

Brochure

Title
New Products Flyer- Vol 4 Signaling Feature- NEW! Phosphohistidine Antibody

Posters

Title
Post-Translational Modifications