Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

06-886-I Anti-NFκB p50 Antibody

Anti-NFκB p50 Antibody is an antibody against NFκB p50 for use in Western Blotting, Immunoprecipitation.

Anti-NFκB p50 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: NFKB1 UniProt Number: P19838
06-886-I
100 μg  
Purchase on Sigma-Aldrich

Oferty specjalne

Przegląd

Replacement Information

Oferty specjalne

100% Performance Guaranteed

Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number06-886-I
DescriptionAnti-NFκB p50 Antibody
Alternate Names
  • Nuclear factor NF-kappa-B p105 subunit
  • NFκB p50
  • DNA-binding factor KBF1
  • EBP-1
  • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
Background InformationNuclear factor NF-kappa-B p105 subunit (UniProt P19838; also known as DNA-binding factor KBF1, EBP-1, NF-kappa-B, NF-kappaB, NF-kB1, NFkappaB, NFKB-p105, NFKB-p50, Nuclear factor NF-kappa-B p50 subunit, Nuclear factor kappa-B DNA binding subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1) is encoded by the NFKB1 gene (Gene ID 4790) in human. NF-κB is a protein complex that plays a key role in regulating the immune response to infection/inflammation. NF-κB1 is synthesized as a 968 a.a protein (p105), which undergoes posttranslational processing to generate the p50 subunit (a.a. 1-433). The p50 NF-κB participates in target gene transactivation by forming heterodimers with RelA, RelB, or c-Rel. In addition, p50 homodimer can bind the nuclear protein Bcl-3 and form a functional transcriptional activator complex. In unstimulated cells, the NF-κB dimers are sequestered in the cytoplasm by the family of IκB (Inhibitor of κB) proteins (IκBα, IκBβ, IκBε, and Bcl-3). Upon stimulation, IκB becomes phosphorylated by the IκB kinase (IKK) complex, leading to IκB ubiquitination and proteasomal degradation. With the degradation of IκB, the NF-κB complex is then freed to enter the nucleus to activate the transcription of target genes.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-NFκB p50 Antibody is an antibody against NFκB p50 for use in Western Blotting, Immunoprecipitation.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis: 10 µg of antibody from a representative lot immunoprecipitated NFkB p50 in 500 µg of A431 cell lysate.
Biological Information
ImmunogenKLH conjugated synthetic peptide (MAEDDPYLGRPE-C) corresponding to amino acids 1-12 of NFκB p50, containing a C-terminal cysteine.
EpitopeAmino acid 1-12
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityDectects 105 kDa recursor and 50 kDa processed forms. Immunogen sequence is a.a. 1-12, this polyclonal antibody can therefore detect isoforms 1 and 2, but not 3. Note that isoform 3 is missing amino acids 1-180.
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Rhesus Macaque based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • NFKB1
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight~50/105 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected NFkB p50 in 10 µg of A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
06-886-I 04055977299915