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ABD135 Anti-NANOS3 Antibody

Detect Nanos homolog 3 using this rabbit polyclonal antibody, Anti-NANOS3 Antibody validated for use in western blotting, IHC (Paraffin) & Immunofluorescence.

Anti-NANOS3 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Pluripotent & Early Differentiation Gene Symbol: NANOS3, Nanos3 UniProt Number: P60323
ABD135
100 µg  
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Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P), IFRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABD135
DescriptionAnti-NANOS3 Antibody
Alternate Names
  • Nanos homolog 3
  • NOS-3
  • NOS3
Background InformationNanos homolog 3 (Nanos3), also known as NOS-3 or NOS3, belongs to the Nanos family. Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. Nanos3 has been shown to play an important role in the maintenance of the undifferentiated state of germ cells regulating the spermatogonia cell cycle and inducing a prolonged transit in G1 phase. Nanos3 has also been shown to be essential in the early stage embryo protecting the migrating primordial germ cells (PGCs) from apoptosis. Additionally, it is implied to maintain the germ cell lineage by suppressing both Bax-dependent and -independent apoptotic pathways and to affect cell proliferation by repressing translation of specific mRNAs. Nanos3 is expressed primarily in ovary, testis and brain. In the ovaries, Nanos3 is expressed during multiple stages of oogenesis, including primordial, primary, secondary and antral follicles with the highest expression in the oocytes. In the testis, Nonos3 is expressed in germ cells, type A spermatogonia (SA), primary spermatocytes (S1), round spermatids (S3) and elongated spermatids.
References
Product Information
FormatAffinity Purified
Control
  • Human brain tissue lysate
PresentationPurified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Nanos homolog 3 using this rabbit polyclonal antibody, Anti-NANOS3 Antibody validated for use in western blotting, IHC (Paraffin) & Immunofluorescence.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
  • Immunofluorescence
Application NotesImmunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected NANOS3 in human brain tissue.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected NANOS3 in human brain cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human NANOS3.
EpitopeC-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes the C-terminus of NANOS3.
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Gene Symbol
  • NANOS3
  • Nanos3
Purification MethodAntigen Affinity Purified
UniProt Number
Molecular Weight~21 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 2 isoforms at ~19 kDa and ~21 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected NANOS3 in 15 µg of human brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
ABD135 04053252912252