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AB6010 Anti-MMP-12 Antibody, NT (active)

Anti-MMP-12 Antibody, N-term (active) is an antibody against MMP-12 for use in WB.

Anti-MMP-12 Antibody, NT (active) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: MMPs & TIMPs Gene Symbol: MMP12, MMP-12, MME, ME, HME UniProt Number: P39900
AB6010
100 µg  
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Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB6010
ReplacesAB19052
DescriptionAnti-MMP-12 Antibody, NT (active)
Alternate Names
  • Macrophage elastase
  • Matrix metalloproteinase-12
  • macrophage metalloelastase
  • matrix metallopeptidase 12
Background InformationMatrix metallopeptidase 12 (MMP-12) is able to degrade elastin, entactin, laminin-1, fibronectin, type IV collagen as well as insulin B-chain and casein. MMP-12 is structurally similar to the classical MMPs (MMP-1, MMP-3); it contains a propeptide with autoinhibitory cysteine switch site, a well conserved zinc site, hinge region and hemopexin domain. MMP-12 lacks a transmembrane domain and furin cleavage site. The zymogen for MMP-12 is about 54 kDa, and is quickly activated to the 45 kDa form, and this breaks down to cascade of active forms, ending with the common 22 kDa form.Stimulated macrophages produce MMP-12; it has also been found in osteosarcoma cells, synovial fibroblasts and lung fibroblasts. MMP-12 levels in quiescent cells and tissues are minimal (with the exception of macrophages), and mitogen stimulation or protein concentration is often needed to visualize the bands. In addition, cell types differ greatly in the quantity of MMP-12 produced.
References
Product Information
FormatAffinity Purified
Control
  • Concentrated conditioned cell culture media collected from a proprietary human fibroblast cell line.
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-MMP-12 Antibody, N-term (active) is an antibody against MMP-12 for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated synthetic peptide corresponding to human MMP-12 near the N-terminus.
EpitopeN-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes MMP-12 near the N-terminus of the active form.
Species Reactivity
  • Human
Species Reactivity NoteHuman. Sequence homology to mouse is 84%.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryProteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
Gene Symbol
  • MMP12
  • MMP-12
  • MME
  • ME
  • HME
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.

COFACTOR: Binds 4 calcium ions per subunit. & Binds 2 zinc ions per subunit.

SIZE: 470 amino acids; 54002 Da

TISSUE SPECIFICITY: Found in alveolar macrophages but not in peripheral blood monocytes.

DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains.
Molecular WeightThe zymogen for MMP-12 is ~ 54 kDa, and is quickly activated to a 45 kDa form. This breaks down to a cascade of active forms, ending with the common 22 kDa form.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot using a concentrated conditioned cell culture media collected from a proprietary human fibroblast cell line (Cat. No. AG902) and probing with 1 µg/mL of Anti-MMP-12.

Western Blot Analysis: 1 µg/ml of this antibody detected MMP-12 on 10 µg of lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
AB6010 04053252740473

Documentation

Anti-MMP-12 Antibody, NT (active) MSDS

Title

Safety Data Sheet (SDS) 

Anti-MMP-12 Antibody, NT (active) Certificates of Analysis

TitleLot Number
Anti-MMP-12, N-term (active) - 2436369 2436369
Anti-MMP-12, N-term (active) - 2033660 2033660
Anti-MMP-12, N-term (active) - 2829138 2829138
Anti-MMP-12, N-term (active) - 3826052 3826052
Anti-MMP-12, N-term (active) - 4006706 4006706
Anti-MMP-12, N-term (active) - NG1740764 NG1740764
Anti-MMP-12, N-term (active) - NG1867626 NG1867626
Anti-MMP-12, N-term (active) - NRG1671160 NRG1671160
Anti-MMP-12, N-term (active) -2743625 2743625
Anti-MMP-12, N-term (active) -2853589 2853589
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