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ABC157 Anti-MCM7 Antibody

This MCM7 antibody is validated for use in WB for the detection of the MCM7 protein.

Anti-MCM7 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: MCM7, CDC47, MCM2 UniProt Number: P33993
ABC157
100 µg  
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Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, Rhesus Macaque, ChpWBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABC157
ReplacesMABE188
DescriptionAnti-MCM7 Antibody
Alternate Names
  • DNA replication licensing factor MCM7
  • CDC47 homolog
  • P1.1-MCM3
Background InformationMCM7 is a highly conserved mini-chromosome maintenance protein (MCM) that is involved in the initiation of eukaryotic genome replication. It forms a hexameric complex with MCM4 and MCM6 (two subunits of MCM7, two subunits of MCM4 and two subunits of MCM7) and this complex regulates the helicase activity of the pre-replication complex. This complex is also involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. MCM7 is reported to bind to DNA in metaphase II in oocytes and remains associated with the DNA until the S-phase. Two isoforms of MCM7 have been reported, 61 kDa and 90 kDa. In neuroblastoma tumors MCM7 expression is shown to correlates with MYCN (v-myc myelocytomatosis viral related oncogene, neuroblastoma derived) amplification where in MCM7 is rapidly up-regulated by Inducible MYCN Expression. MYCN protein is shown to bind specifically to the MCM7 promoter region, which possesses an E-box binding site for the Myc oncoproteins.
References
Product Information
FormatPurified
Control
  • HeLa cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis MCM7 antibody is validated for use in WB for the detection of the MCM7 protein.
Key Applications
  • Western Blotting
Application NotesWestern Blot Analysis: 0.5 µg/mL from a representative lot detected MCM7 in 10 µg of L6 cell lysate.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human MCM7.
EpitopeN-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes the N-terminus of MCM7
Species Reactivity
  • Human
  • Rat
  • Rhesus Macaque
  • Chimpanzee
Species Reactivity NoteDemonstrated to react with Human and Rat. Predicted to react with Rhesus Macaque and Chimpanzee based on 100% sequence homology. Other homologies: Mouse (71% sequence homology).
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • MCM7
  • CDC47
  • MCM2
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight~82 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected MCM7 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
ABC157 04053252830945