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MAB1614 Anti-Kinesin Antibody, heavy chain, a.a.420-445, clone H2

MAB1614
100 µg  
Purchase on Sigma-Aldrich

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Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
Ch, Ma, Sqd, Su, H, M, RELISA, ICC, IHC, IP, FUNC, RIA, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB1614
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Kinesin Antibody, heavy chain, a.a.420-445, clone H2
Background InformationKinesins are molecular motors used for intracellular transport and trafficking. They move along microtubule cables powered by the hydrolysis of ATP, thus kinesins are ATPases. The active movement of kinesins supports several cellular functions including mitosis, meiosis and transport of cargo such as axonal transport.
References
Product Information
FormatPurified
Control
  • Brain tissue
PresentationPurified mouse monoclonal IgG2b in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Kinesin using this Anti-Kinesin Antibody, heavy chain, a.a.420-445, clone H2 validated for use in ELISA, IC, IH, IP, FUNC, RIA & WB.
Key Applications
  • ELISA
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunoprecipitation
  • Affects Function
  • Radioimmunoassay
  • Western Blotting
Application NotesImmunohistochemistry:
A previous lot was used on paraformaldehyde fixed tissue. Also works for EM.

Immunocytochemistry:
A previous lot of this antibody was used in Immunocytochemistry.

Immunoprecipitation:
A previous lot of this antibody was used in Immunoprecipitation.

EIA/RIA:
A previous lot of this antibody was used in RIA.

Immunoblotting: recognizes 124kDa KHC and its proteolytic fragments.

Inhibition of kinesin mediated transport {Brady, et al PNAS 87(3):1061-1065, 1990.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenBovine brain kinesin.
Epitopea.a.420-445
CloneH2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityReacts with the heavy chain of kinesin near the N-terminus.
IsotypeIgG2b
Species Reactivity
  • Chicken
  • Mammals
  • Squid
  • Sea Urchin
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • KIF5B
  • UKHC
  • KINH
  • KNS1
  • KNS
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P33176 # Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes (By similarity).
SIZE: 963 amino acids; 109685 Da
SUBUNIT: Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1 (By similarity). Interacts with SYBU.
SUBCELLULAR LOCATION: Note=Uniformly distributed between soma and neurites in hippocampal neurons (By similarity).
DOMAIN: SwissProt: P33176 Composed of three structural domains: a large globular N- terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
SIMILARITY: Belongs to the kinesin-like protein family. Kinesin subfamily. & Contains 1 kinesin-motor domain.
Molecular Weight124 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot on Mouse Brain lysates.

Western Blot Analysis:
1:500 dilution of this lot detected KINESIN (HEAVY CHAIN) on 10 μg of Mouse Brain lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
MAB1614 04053252727566

Documentation

Anti-Kinesin Antibody, heavy chain, a.a.420-445, clone H2 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Kinesin Antibody, heavy chain, a.a.420-445, clone H2 Certificates of Analysis

TitleLot Number
Anti-Kinesin, heavy chain, a.a.420- -2739313 2739313
Anti-Kinesin, heavy chain, a.a.420- 445, clone H2 - 1997923 1997923
Anti-Kinesin, heavy chain, a.a.420- 445, clone H2 - 2069356 2069356
Anti-Kinesin, heavy chain, a.a.420- 445, clone H2 - 2210350 2210350
Anti-Kinesin, heavy chain, a.a.420- 445, clone H2 - LV1459899 LV1459899
Anti-Kinesin, heavy chain, a.a.420- 445, clone H2 - LV1594756 LV1594756
Anti-Kinesin, heavy chain, a.a.420- 445, clone H2 - LV1691087 LV1691087
Anti-Kinesin, heavy chain, a.a.420- 445, clone H2 - LV1777794 LV1777794
Anti-Kinesin, heavy chain, a.a.420- 445, clone H2 - NG1842893 NG1842893
Anti-Kinesin, heavy chain, a.a.420- 445, clone H2 - NG1940109 NG1940109

References

Reference overviewApplicationPub Med ID
A method for multiprotein assembly in cells reveals independent action of kinesins in complex.
Norris, SR; Soppina, V; Dizaji, AS; Schimert, KI; Sept, D; Cai, D; Sivaramakrishnan, S; Verhey, KJ
The Journal of cell biology  207  393-406  2014

Pokaż streszczenie
25365993 25365993
Recycling of kinesin-1 motors by diffusion after transport.
Blasius, TL; Reed, N; Slepchenko, BM; Verhey, KJ
PloS one  8  e76081  2013

Pokaż streszczenie
24098765 24098765
Opposing microtubule motors drive robust nuclear dynamics in developing muscle cells.
Wilson, MH; Holzbaur, EL
Journal of cell science  125  4158-69  2011

Pokaż streszczenie
22623723 22623723
Distinct mechanisms of axonal globule formation in mice expressing human wild type α-synuclein or dementia with Lewy bodies-linked P123H β-synuclein.
Sekigawa, A; Fujita, M; Sekiyama, K; Takamatsu, Y; Hatano, T; Rockenstein, E; La Spada, AR; Masliah, E; Hashimoto, M
Molecular brain  5  34  2011

Pokaż streszczenie
23013868 23013868
RNA interference of Marlin-1/Jakmip1 results in abnormal morphogenesis and migration of cortical pyramidal neurons.
Ren Vidal,Patricio Fuentes,Jos Valenzuela,Carlos P Alvarado-Diaz,Omar A Ram,Manuel Kukuljan,Andr Couve
Molecular and cellular neurosciences  51  2011

Pokaż streszczenie
22828129 22828129
A non-mammalian type opsin 5 functions dually in the photoreceptive and non-photoreceptive organs of birds.
Ohuchi, H; Yamashita, T; Tomonari, S; Fujita-Yanagibayashi, S; Sakai, K; Noji, S; Shichida, Y
PloS one  7  e31534  2011

Pokaż streszczenie
22348098 22348098
In vitro motility of liver connexin vesicles along microtubules utilizes kinesin motors.
Fort, AG; Murray, JW; Dandachi, N; Davidson, MW; Dermietzel, R; Wolkoff, AW; Spray, DC
The Journal of biological chemistry  286  22875-85  2010

Pokaż streszczenie
21536677 21536677
KASH protein Syne-2/Nesprin-2 and SUN proteins SUN1/2 mediate nuclear migration during mammalian retinal development.
Yu, J; Lei, K; Zhou, M; Craft, CM; Xu, G; Xu, T; Zhuang, Y; Xu, R; Han, M
Human molecular genetics  20  1061-73  2010

Pokaż streszczenie Pełny tekst artykułu
21177258 21177258
Effects of ALS-related SOD1 mutants on dynein- and KIF5-mediated retrograde and anterograde axonal transport.
Ping Shi,Anna-Lena Ström,Jozsef Gal,Haining Zhu
Biochimica et biophysica acta  1802  2009

Pokaż streszczenie Pełny tekst artykułu
20510358 20510358
Mutant SOD1 impairs axonal transport of choline acetyltransferase and acetylcholine release by sequestering KAP3.
Tateno, M; Kato, S; Sakurai, T; Nukina, N; Takahashi, R; Araki, T
Human molecular genetics  18  942-55  2009

Pokaż streszczenie
Western Blotting19088126 19088126