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07-716-I Anti-KDR/Flk-1/VEGFR2 Antibody

Detect KDR/Flk-1/VEGFR2 using this rabbit polyclonal antibody, Anti-KDR/Flk-1/VEGFR2 Antibody validated for use in western blotting.

Anti-KDR/Flk-1/VEGFR2 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: Kdr, Flk-1, Flk1 UniProt Number: P35918
07-716-I
100 µg  
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Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-716-I
Replaces07-716
DescriptionAnti-KDR/Flk-1/VEGFR2 Antibody
Alternate Names
  • Vascular endothelial growth factor receptor 2
  • VEGFR-2
  • Fetal liver kinase 1
  • FLK-1
  • Kinase NYK
  • Protein-tyrosine kinase receptor flk-1
  • CD309
Background InformationThe Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect KDR/Flk-1/VEGFR2 using this rabbit polyclonal antibody, Anti-KDR/Flk-1/VEGFR2 Antibody validated for use in western blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected KDR/Flk-1/VEGFR2 in 10 µg of PAE cells transfected with CSF/VEGFR2 chimera.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the C-terminus of mouse KDR/Flk-1/VEGFR2.
EpitopeC-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Mouse
  • Human
Antibody TypePolyclonal Antibody
Gene Symbol
  • Kdr
  • Flk-1
  • Flk1
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~230 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HUVEC cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected KDR/Flk-1/VEGFR2 in 10 µg of HUVEC cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
07-716-I 04053252937385