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MAB2060 Anti-Integrin β4 Antibody, clone ASC-9

MAB2060
100 µg  
Purchase on Sigma-Aldrich

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Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, IP, IHC, FUNCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB2060
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Integrin β4 Antibody, clone ASC-9
Alternate Names
  • CD104
References
Product Information
FormatPurified
PresentationLiquid in 0.02M PB pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Integrin β4 Antibody, clone ASC-9 is validated for use in FC, IP, IH, FUNC for the detection of Integrin β4.
Key Applications
  • Flow Cytometry
  • Immunoprecipitation
  • Immunohistochemistry
  • Affects Function
Application NotesInhibition of cell attachment: inhibits SCC9 cell adhesion to human and

EHS laminin, but not to laminin GD-2 peptide, type IV collagen or fibronectin. Does not inhibit SKOV-3 cell adhesion to laminin, fibronectin or type IV collagen.

Immunohistochemistry: on acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue.

Flow cytometry: stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelium (HUVEC). Stains RKO or K562 cells transfected for CD104 expression. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets.

Immunoprecipitation: precipitates PAGE bands of 66, 76, 120, 135 and 200kDa (reduced) or 66, 110, 125, 130 and 190kDa (non-reduced) from surface-biotinylated SCC9 cells.

Working dilutions must be determined by end user.
Biological Information
ImmunogenHuman squamous cell carcinoma cells (SCC9)
CloneASC-9
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityHuman beta4 integrin (CD104)
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryIntegrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Gene Symbol
  • ITGB4
  • CD104
  • GP150
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P16144 # Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
SIZE: 1822 amino acids; 202151 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-4 associates with alpha-6.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
DOMAIN: SwissProt: P16144 The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
DISEASE: SwissProt: P16144 # Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus. & Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. This nonlethal form of junctional epidermolysis bullosa is characterized by life- long blistering of the skin, associated with hair and tooth abnormalities.
SIMILARITY: Belongs to the integrin beta chain family. & Contains 1 Calx-beta domain. & Contains 4 fibronectin type-III domains. & Contains 1 VWFA domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 6 months.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
MAB2060 04053252267413

Documentation

Anti-Integrin β4 Antibody, clone ASC-9 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Integrin β4 Antibody, clone ASC-9 Certificates of Analysis

TitleLot Number
MOUSE ANTI-HUMAN INTEGRIN b4 [CD104] MONOCLONAL ANTIBODY - 2188478 2188478
MOUSE ANTI-HUMAN INTEGRIN b4 [CD104] MONOCLONAL ANTIBODY - 2193300 2193300
MOUSE ANTI-HUMAN INTEGRIN b4 [CD104] MONOCLONAL ANTIBODY - 2299961 2299961
MOUSE ANTI-HUMAN INTEGRIN ß4 [CD104] -2858449 2858449
MOUSE ANTI-HUMAN INTEGRIN β4 [CD104] - 3541922 3541922
MOUSE ANTI-HUMAN INTEGRIN β4 [CD104] -2593140 2593140
MOUSE ANTI-HUMAN INTEGRIN β4 [CD104] -2669944 2669944

References

Reference overviewApplicationSpeciesPub Med ID
Role of integrins in the assembly and function of hensin in intercalated cells.
Vijayakumar, S; Erdjument-Bromage, H; Tempst, P; Al-Awqati, Q
Journal of the American Society of Nephrology : JASN  19  1079-91  2008

Pokaż streszczenie Pełny tekst artykułu
Western Blotting, ImmunoprecipitationRabbit18337486 18337486
A novel recognition site on laminin for the alpha 3 beta 1 integrin.
Pattaramalai, S, et al.
Exp. Cell Res., 222: 281-90 (1996)  1996

8598215 8598215
A new DPB1 allele (DPB1*5901) identified by reverse dot blot hybridization and confirmed by DNA sequencing.
Perrier, P and Dormoy, A
Tissue Antigens, 47: 356-9 (1996)  1996

8773330 8773330