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MAB1965 Anti-Integrin β1 Antibody, a.a. 82-87, clone JB1A (a.k.a. J10)

MAB1965
100 µL  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, IHC, IP, FUNC, WBMAscitesMonoclonal Antibody
Description
Catalogue NumberMAB1965
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Integrin β1 Antibody, a.a. 82-87, clone JB1A (a.k.a. J10)
Alternate Names
  • CD29
Background InformationThe primary function of the integrin family is to mediate cell-cell and cell-matrix adhesion interaction. CD29 is ubiquitous, with broad tissue distribution, but is not expressed on erythrocytes and is expressed only weakly on granulocytes. Integrins play an important role in cell adhesion and migration, and their normal function is critical in the induction and maintenance of cell differentiation. Loss or downregulation of CD29 has been proposed to be one of the general pathways through which carcinoma cells may acquire a more invasive and differentiated phenotype. Furthermore, loss or downregulation of integrin occurs relatively frequently in poorly differentiated colorectal and breast carcinomas as determined by immunohistochemistry.
References
Product Information
FormatAscites
HS Code3002 15 90
Control
  • Human lung, liver, and skeletal muscle tissues
PresentationAscites fluid containing no preservatives.
Quality LevelMQ100
Applications
ApplicationThis Anti-Integrin β1 Antibody, a.a. 82-87, clone JB1A (a.k.a. J10) is validated for use in FC, IH, IP, FUNC, WB for the detection of Integrin β1.
Key Applications
  • Flow Cytometry
  • Immunohistochemistry
  • Immunoprecipitation
  • Affects Function
  • Western Blotting
Application NotesInhibition of Jurkat binding to collagen and fibronectin:
1:100 Note: epitope is trypsin sensitive; cells should be prepared with PBS + EDTA (2mM) or other non-enzymatic methods. Blocking will not occur with trypsinized cells with JB1A.

Immunoprecipitation: 1:250-500

Western blotting (reducing, 115 kDa; non-reducing, 130 kDa): 1:1000-2000

Note: binding of antibody may be inhibited by magnesium ion concentrations exceeding 0.5mM.

Immunocytochemistry/Immunohistochemistry: JB1A can be used on unfixed cells in flow cytometry and culture {Akimov, 2001; Blood 98(5):1567-1576}. Often times fixing with 2-3% PFA for 5 minutes after primary antibody incubation will assist in obtain strong signals since it fixes the primary antibodies to the tissues.

FACS: JB1A has been primarily used against live cells at 1:200-1:500 followed by goat anti-mouse conjugated secondaries

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenPurified beta 1 integrin and Jurkat cells
Epitopea.a. 82-87
CloneJB1A (a.k.a. J10)
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityReacts with the human integrin beta 1. Note: integrin beta 1 is expressed in all tissues/cell types except erythrocytes. The epitope recognized by JB1a was recently mapped to residues 82-87 of the mature beta 1 integrin chain. In this study Mn++ was found to inhibit binding of JB1a both to mature beta 1 integrin and to a synthetic peptide corresponding to residues 82-87{Ni, 1998}.
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryIntegrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.
Gene Symbol
  • ITGB1
  • GPIIA
  • OTTHUMP00000063731
  • OTTHUMP00000046253
  • OTTHUMP00000063733
  • VLAB
  • OTTHUMP00000063732
  • MDF2
  • FNRB
  • CD29
  • MSK12
Purification MethodUnpurified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P05556 # Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta- 1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha- 1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha- 3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha- 10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.


SIZE: 798 amino acids; 88465 Da

SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-1 associates with either alpha-1, alpha-2, alpha-3, alpha-4, alpha- 5, alpha-6, alpha-7, alpha-8, alpha-9, alpha-10, alpha-11 or alpha-V. Interacts with FLNA. Binds LGALS3BP and ITGB1BP3, when associated with alpha-7, but not with alpha-5 (By similarity). Interacts with FLNB and RANBP9. Isoform Beta-1D interacts with ACE2. Isoform Beta-1A interacts with the C-terminal region of FLNC. Interacts with HIV-1 Tat.

SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Melanosome. Note=Isoform beta-1B does not localize to focal adhesions. Highly enriched in stage I melanosomes.

TISSUE SPECIFICITY: Isoform beta-1A is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform beta-1B is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbelical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform beta-1C and isoform beta-1C-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-C-2, rather than isoform beta-1C, is selectively expressed in primary T-cells. Isoform beta-1C is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1D is expressed specifically in striated muscle (skeletal and cardiac muscle).





SIMILARITY: SwissProt: P05556 ## Belongs to the integrin beta chain family. & Contains 1 VWFA domain.
Molecular Weight88 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
MAB1965 08436037124761

Documentation

Anti-Integrin β1 Antibody, a.a. 82-87, clone JB1A (a.k.a. J10) MSDS

Title

Safety Data Sheet (SDS) 

Anti-Integrin β1 Antibody, a.a. 82-87, clone JB1A (a.k.a. J10) Certificates of Analysis

TitleLot Number
MOUSE ANTI-HUMAN INTEGRIN beta1 2474973
MOUSE ANTI-HUMAN INTEGRIN beta1 MONOCLONAL ANTIBODY - 2118036 2118036
MOUSE ANTI-HUMAN INTEGRIN beta1 MONOCLONAL ANTIBODY - 2420448 2420448
MOUSE ANTI-HUMAN INTEGRIN beta1 - 2510323 2510323
MOUSE ANTI-HUMAN INTEGRIN beta1 - 3484999 3484999
MOUSE ANTI-HUMAN INTEGRIN beta1 - 3776181 3776181
MOUSE ANTI-HUMAN INTEGRIN beta1 - 4131158 4131158
MOUSE ANTI-HUMAN INTEGRIN beta1 -2551195 2551195
MOUSE ANTI-HUMAN INTEGRIN beta1 -2583382 2583382
MOUSE ANTI-HUMAN INTEGRIN beta1 -2594385 2594385

References

Reference overviewApplicationSpeciesPub Med ID
Slingshot-Cofilin activation mediates mitochondrial and synaptic dysfunction via Aβ ligation to β1-integrin conformers.
Woo, JA; Zhao, X; Khan, H; Penn, C; Wang, X; Joly-Amado, A; Weeber, E; Morgan, D; Kang, DE
Cell death and differentiation  22  921-34  2015

Pokaż streszczenie
25698445 25698445
Prestress in the extracellular matrix sensitizes latent TGF-β1 for activation.
Klingberg, F; Chow, ML; Koehler, A; Boo, S; Buscemi, L; Quinn, TM; Costell, M; Alman, BA; Genot, E; Hinz, B
The Journal of cell biology  207  283-97  2014

Pokaż streszczenie
25332161 25332161
Recombinant viral capsid protein VP1 suppresses lung cancer metastasis by inhibiting COX-2/PGE2 and MIG-7.
Ho, MY; Hung, SW; Liang, CM; Liang, SM
Oncotarget  5  3931-43  2014

Pokaż streszczenie
25004182 25004182
Recombinant viral capsid protein VP1 suppresses migration and invasion of human cervical cancer by modulating phosphorylated prohibitin in lipid rafts.
Ching-Feng Chiu,Jei-Ming Peng,Shao-Wen Hung,Chi-Ming Liang,Shu-Mei Liang
Cancer letters  320  2011

Pokaż streszczenie
22388104 22388104
Recombinant viral protein promotes apoptosis and suppresses invasion of ovarian adenocarcinoma cells by targeting α5β1 integrin to down-regulate Akt and MMP-2.
Jei-Ming Peng,Yee-Hsiung Chen,Shao-Wen Hung,Ching-Feng Chiu,Ming-Yi Ho,Yi-Jen Lee,Tsung-Ching Lai,Michael Hsiao,Chi-Ming Liang,Shu-Mei Liang
British journal of pharmacology  165  2011

Pokaż streszczenie
21740408 21740408
The MUC1 extracellular domain subunit is found in nuclear speckles and associates with spliceosomes.
Kumar, P; Kumar, P; Lindberg, L; Thirkill, TL; Ji, JW; Martsching, L; Douglas, GC
PloS one  7  e42712  2011

Pokaż streszczenie
22905162 22905162
Integrin β1 subunit signaling is involved in the directed migration of human retinal pigment epithelial cells following electric field stimulation.
Jing Han,Xiao-Long Yan,Quan-Hong Han,Yang-Jun Li,Zhao-Jiang Du,Yan-Nian Hui
Ophthalmic research  45  2010

Pokaż streszczenie
20720436 20720436
Differential roles of endothelin-1 in angiotensin II-induced atherosclerosis and aortic aneurysms in apolipoprotein E-null mice.
Suen, RS; Rampersad, SN; Stewart, DJ; Courtman, DW
The American journal of pathology  179  1549-59  2010

Pokaż streszczenie
21718678 21718678
Endothelial cell adhesion to the extracellular matrix induces c-Src-dependent VEGFR-3 phosphorylation without the activation of the receptor intrinsic kinase activity.
Galvagni, F; Pennacchini, S; Salameh, A; Rocchigiani, M; Neri, F; Orlandini, M; Petraglia, F; Gotta, S; Sardone, GL; Matteucci, G; Terstappen, GC; Oliviero, S
Circulation research  106  1839-48  2009

Pokaż streszczenie
20431062 20431062
Curcumin mediated suppression of nuclear factor-κB promotes chondrogenic differentiation of mesenchymal stem cells in a high-density co-culture microenvironment.
Buhrmann, C; Mobasheri, A; Matis, U; Shakibaei, M
Arthritis research & therapy  12  R127  2009

Pokaż streszczenie
20594343 20594343