Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABF222 Anti-IKKa (p45) Antibody, clone 881H3

This Anti-IKKa (p45) Antibody, clone 881H3 is validated for use in western blotting, IHC (Paraffin) & IP for the detection of IKKa (p45).

Anti-IKKa (p45) Antibody, clone 881H3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Inflammation & Autoimmune Mechanisms Gene Symbol: CHUK, IKKA, TCF16 UniProt Number: O15111
MABF222
100 µg  
Purchase on Sigma-Aldrich

Oferty specjalne

Przegląd

Replacement Information

Oferty specjalne

100% Performance Guaranteed

Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P), IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF222
DescriptionAnti-IKKa (p45) Antibody, clone 881H3
Alternate Names
  • Inhibitor of nuclear factor kappa-B kinase subunit alpha
  • I-kappa-B kinase alpha
  • IKK-A
  • IKK-alpha
  • IkBKA
  • IkappaB kinase
  • Conserved helix-loop-helix ubiquitous kinase
  • I-kappa-B kinase 1
  • IKK1
  • Nuclear factor NF-kappa-B inhibitor kinase alpha
  • NFKBIKA
  • Transcription factor 16
  • TCF-16
Background InformationThe protein named Inhibitor of nuclear factor kappa-B kinase subunit alpha, or alternatively I-kappa-B kinase alpha, IKK-A, IKK-alpha, IKKa (p45), IkBKA, IkappaB kinase, I-kappa-B kinase 1, IKK1, NF-kappa-B inhibitor kinase alpha, NFKBIKA, or transcription factor 16 (TCF-16) and encoded by the human gene CHUK/IKKA/TCF16 is a serine kinase that plays an essential role in the NF-kappa-B signaling pathway. IKKa(p45) acts as part of a complex that phosphorylates inhibitors of the NF-kappa-B. This allows then the polyubiquitination of the inhibitors and their subsequent degradation. Free NF-kappa-B is translocated into the nucleus and performs its gene activation functions governing everything from the immune response to growth and cell survival. IKKa(p45) can be found expressed in most tissues and localizes to both the cytoplasm and nucleus depending upon the microenvironment. Defects in IKKa(p45) are associated with a lethal fetal syndrome called by Cocoon syndrome where the fetus has multiple malformations including limbs bound to the trunk and encased under the skin.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-IKKa (p45) Antibody, clone 881H3 is validated for use in western blotting, IHC (Paraffin) & IP for the detection of IKKa (p45).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected IKKa (p45) in human kidney and tonsil tissues.

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated IKKa (p45) from HCT116 cell lysate (Margalef, P., et al. (2012). Cell Rep. 2(40):840-854).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected IKKa (p45) in human intestinal mucosa, adenoma, and carcinoma tissues (Margalef, P., et al. (2012). Cell Rep. 2(40):840-854.).

Western Blotting Analysis: A previous lot of this antibody detected the truncated p45-IKKa form observed at ~45 kDa in WT MEF cell lysate, but less so in the KO MEFs, and the full length non-denatured protein at ~80-85 kDa. A non-specific band at ~75 kDa is detected.

Immunoprecipitation Analysis: A previous lot of this antibody immunoprecipiated the truncated p45-IKKa form observed at ~45 kDa.
Biological Information
ImmunogenConjugated linear peptide corresponding to human IKKa (p45).
Clone881H3
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody preferentially binds both the p45 fragment of IKKa (~45 kDa) as well as the full-length non-denatured form (~80-85 kDa). This antibody shows better preferential binding to the p45 fragment by immunoprecipitation, and detects both forms of IKKa by western blot.
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CHUK
  • IKKA
  • TCF16
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~80-85 kDa & ~45 kDa observed. The full length non-denatured protein may be observed at ~80-85 kDa, whereas the truncated p45-IKKa fragment may be observed at ~45 kDa in some lysates. A non-specific band at ~75 kDa may also be observed in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HCT116 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected IKKa (p45) in 10 µg of HCT116 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
MABF222 04053252946912

Documentation

Anti-IKKa (p45) Antibody, clone 881H3 MSDS

Title

Safety Data Sheet (SDS) 

Anti-IKKa (p45) Antibody, clone 881H3 Certificates of Analysis

TitleLot Number
Anti-IKKa (p45), clone 881H3 - 3270714 3270714
Anti-IKKa (p45), clone 881H3 - 3560022 3560022
Anti-IKKa (p45), clone 881H3 - 3928119 3928119
Anti-IKKa (p45), clone 881H3 - Q2365772 Q2365772

References

Reference overviewPub Med ID
A truncated form of IKKα is responsible for specific nuclear IKK activity in colorectal cancer.
Margalef, Pol, et al.
Cell Rep, 2: 840-54 (2012)  2011

Pokaż streszczenie 		23041317 23041317