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OB05 Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2)

Anti-Histidine-Tagged Protein, mouse monoclonal, clone 13/45/31/2, specifically recognizes an epitage of 6 consecutive His residues. It is validated for use in ELISA, WB, IF & IP.

Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-His6

OB05
Purchase on Sigma-Aldrich

Przegląd

Replacement Information

Tabela kluczowych gatunków

Species ReactivityHostAntibody Type
AMMonoclonal Antibody

Products

Numer katalogowyOpakowanie Ilość/opak.
OB05-100UG Ampulka plastikowa 100 μg
Description
OverviewSpecifically recognizes an epitage of six consecutive His residues of both natural and recombinant sources.
Catalogue NumberOB05
Brand Family Calbiochem®
SynonymsAnti-His6
Application Data
Detection of human histidine-tagged protein by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2) (Cat. No. OB05) (1:100). Detection: chemiluminescence.

Detection of human histidine-tagged protein by immunofluorescence. Samples: Transfected HeLa cells from the plasmid expression pCMV-His6-GFP-APKD1-5a with GFP autofluorescence (left figure) and primary antibody histidine-tagged protein, secondary antibody Cy3-conjugated anti-mouse (right figure). Primary antibody: Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2) (Cat. No. OB05) (1:100). Detection: fluorescence.
References
ReferencesZentgraf, H., et al. 1995. Nucl. Acids. Res. 23, 3347.
Gu, J., et al. 1994. BioTechniques 17, 257.
Sporeno, E., et al. 1994. J. Biol. Chem., 10991.
Sisk, W.P., et al. 1994. J. Virol. 68, 766.
Lu, T., et al. 1993. Anal. Biochem. 213, 318.
Garner, J., et al. 1992. Cell 69, 833.
Hochuli, E., et al. 1987. J. Chromatogr. 411, 177.
Product Information
DeclarationManufactured by Dianova, GmbH, Germany.
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Positive controlProteins containing a His•Tag® sequence
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Application NotesELISA (0.1-0.4 µg/ml)
Immunoblotting (1-4 µg/ml)
Immunofluorescence (1-4 µg/ml)
Immunoprecipitation (4 µg/ml)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena recombinant protein containing a His•Tag® sequence
EpitopeHHHHHH
Clone13/45/31/2
HostMouse
IsotypeIgG₁
Species Reactivity
  • All
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
OB05-100UG 04055977210057

Documentation

Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2) MSDS

Title

Safety Data Sheet (SDS) 

Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2) Certificates of Analysis

TitleLot Number
OB05

References

Przegląd literatury
Zentgraf, H., et al. 1995. Nucl. Acids. Res. 23, 3347.
Gu, J., et al. 1994. BioTechniques 17, 257.
Sporeno, E., et al. 1994. J. Biol. Chem., 10991.
Sisk, W.P., et al. 1994. J. Virol. 68, 766.
Lu, T., et al. 1993. Anal. Biochem. 213, 318.
Garner, J., et al. 1992. Cell 69, 833.
Hochuli, E., et al. 1987. J. Chromatogr. 411, 177.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-January-2009 JSW
SynonymsAnti-His6
ApplicationELISA (0.1-0.4 µg/ml)
Immunoblotting (1-4 µg/ml)
Immunofluorescence (1-4 µg/ml)
Immunoprecipitation (4 µg/ml)
Application Data
Detection of human histidine-tagged protein by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2) (Cat. No. OB05) (1:100). Detection: chemiluminescence.

Detection of human histidine-tagged protein by immunofluorescence. Samples: Transfected HeLa cells from the plasmid expression pCMV-His6-GFP-APKD1-5a with GFP autofluorescence (left figure) and primary antibody histidine-tagged protein, secondary antibody Cy3-conjugated anti-mouse (right figure). Primary antibody: Anti-Histidine-Tagged Protein Mouse mAb (13/45/31/2) (Cat. No. OB05) (1:100). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes a His•Tag® sequence in native and recombinant proteins. Specifically recognizes an epitope of six consecutive His residues of both natural and recombinant sources.
BackgroundIdentification of proteins for which there does not exist antibodies has traditionally been overcome by the use of epitope tagging systems in which expression vectors containing an open reading frame encoding a small fragment of a specific protein (for which an antibody exists) together with multiple cloning sites are used to express foreign genes. Several different epitope tagging systems of this type currently in use include maltose-binding-protein (MBP), glutathione S-transferase (GST), TrpE, and myc. Tags are generally placed at either the N-or C-terminus of the protein as epitope tags placed within a coding sequence of a target protein increases the possibility of interference with the function of the protein and also decreases ability of tag-specific antibody to detect epitope tagged sequence buried by native folding of the target protein. A second type of epitope tagging involves the addition of multiple histidine residues, usually six residues, at either the N- or C-terminus of the target protein. Expressed proteins with such a tag may then be purified by binding to divalent metal ion columns, most commonly to chelated-Ni2+ columns using a technique known as IMAC or immobilized metal ion chromatography. This type of epitope tagging is particularly suited for the purification of recombinant proteins from E. coli expression systems. Analysis of histidine-tagged proteins by immunoblotting or immunofluorescence may also be done using an antibody to the histidine tag itself, thereby eliminating the need for an antibody specific for the protein in question.
HostMouse
Immunogena recombinant protein containing a His•Tag® sequence
EpitopeHHHHHH
Clone13/45/31/2
IsotypeIgG₁
Speciesall His•Tag® proteins
Positive controlProteins containing a His•Tag® sequence
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesZentgraf, H., et al. 1995. Nucl. Acids. Res. 23, 3347.
Gu, J., et al. 1994. BioTechniques 17, 257.
Sporeno, E., et al. 1994. J. Biol. Chem., 10991.
Sisk, W.P., et al. 1994. J. Virol. 68, 766.
Lu, T., et al. 1993. Anal. Biochem. 213, 318.
Garner, J., et al. 1992. Cell 69, 833.
Hochuli, E., et al. 1987. J. Chromatogr. 411, 177.