Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

06-1294 Anti-HCE Antibody

Anti-HCE Antibody is a Sheep Polyclonal Antibody for detection of HCE also known as RNA guanylyltransferase & 5'-phosphatase & has been validated in WB.

Anti-HCE Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: CAP1A, DKFZp686J2031, HCAP1, HCE , HCE1, OTTHUMP00000016841, hCAP UniProt Number: O60942
06-1294
100 µl  
Purchase on Sigma-Aldrich

Oferty specjalne

Przegląd

Replacement Information

Oferty specjalne

100% Performance Guaranteed

Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWBShUnpurifiedPolyclonal Antibody
Description
Catalogue Number06-1294
DescriptionAnti-HCE Antibody
Alternate Names
  • RNA guanylyltransferase and 5'-phosphatase
Background InformationThe HCE protein, also known as RNA guanylyltransferase and 5'-phosphatase is a bifunctional enzyme. In cells this protein functions as a mRNA-capping enzyme with mRNA guanylyltransferase activity in the C-terminal region and RNA 5'-triphosphatase activity in the N-terminal region. This protein catalyzes the first two steps of cap formation: removal of the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and transfer of the gmp moiety of GTP to the 5'-diphosphate terminus.
References
Product Information
FormatUnpurified
Control
  • Human HCE protein
PresentationUnpurified sheep polyclonal in serum with 0.05% sodium azide.
Applications
ApplicationAnti-HCE Antibody is a Sheep Polyclonal Antibody for detection of HCE also known as RNA guanylyltransferase & 5'-phosphatase & has been validated in WB.
Key Applications
  • Western Blotting
Biological Information
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostSheep
SpecificityThis antibody recognizes HCE.
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CAP1A
  • DKFZp686J2031
  • HCAP1
  • HCE
  • HCE1
  • OTTHUMP00000016841
  • hCAP
Purification MethodUnpurified
UniProt Number
UniProt SummaryFUNCTION: Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.
CATALYTIC ACTIVITY: A 5'-phosphopolynucleotide + H2O = a polynucleotide + phosphate.
GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
SUBUNIT STRUCTURE: Interacts with SUPT5H and RNMT. Interacts with HIV-1 Tat.
SUBCELLULAR LOCATION: Nucleus By similarity.
TISSUE SPECIFICITY: Isoform 1 and isoform 4 (at a lesser extent) are expressed in cerebrum, cerebellum, thyroid, lung, heart, liver, kidney, spleen, large intestine, testis, skin and muscle.
MISCELLANEOUS: Isoform 2 to isoform 4 lack mRNA 5'-guanylyltransferase activity due to disruptions of the GTase domain.
SEQUENCE SIMILARITIES: In the N-terminal section; belongs to the non-receptor class of the protein-tyrosine phosphatase family.
In the C-terminal section; belongs to the eukaryotic GTase family.
Molecular Weight~68 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in human HCE protein.
Western Blot Analysis: A 1:1000 dilution of this antibody detected HCE on 10 µg of human HCE protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µl
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
06-1294 04053252670954

Documentation

Anti-HCE Antibody Certificates of Analysis

TitleLot Number
Anti-HCE - NRG1677511 NRG1677511