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ABS1601 Anti-GRASP65 Antibody

Anti-GRASP65 Antibody is an antibody against GRASP65 for use in Western Blotting, Immunoprecipitation, Immunocytochemistry.

Anti-GRASP65 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: Gorasp1, Grasp65 UniProt Number: O35254
ABS1601
100 µL  
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Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, IP, ICCRbSerumPolyclonal Antibody
Description
Catalogue NumberABS1601
DescriptionAnti-GRASP65 Antibody
Alternate Names
  • Golgi reassembly-stacking protein 1
  • GRASP65
  • Golgi peripheral membrane protein p65
  • Golgi reassembly-stacking protein of 65 kDa
Background InformationGolgi reassembly stacking protein of 65 kDa (also called Golgi reassembly and stacking protein 1 or Golgi peripheral membrane protein p65) is encoded by the Grasp65 or Gorasp65 gene in rat (GRASP65, GORASP1, or GOLPH5 gene in human). It is is a Golgi structural protein and mediates cisternae stacking via self-aggregation as well as interactions with other Golgi membrane-docking proteins, such as GM130. GRASP65 dimers are shown to aggregate in vitro and form extensive trans oligomers, which disaggregate upon phosphorylation by cdc2-cyclin B and polo-like kinases, and re-aggregate upon dephosphorylation (PMID 12839990). Fluorescent imaging studies reveal a dynamic exchange between the membrane-associated and cytosolic GRASP65. (PMID 11706049).
References
Product Information
FormatSerum
PresentationRabbit polyclonal serum containing 0.05% sodium azide.
Quality LevelMQ300
Applications
ApplicationAnti-GRASP65 Antibody is an antibody against GRASP65 for use in Western Blotting, Immunoprecipitation, Immunocytochemistry.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunocytochemistry
Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated GRASP65 in APPswe/PS1ΔE9 cells stably expressing GFP-tagged GRASP65 WT (Joshi, G., et al. (2013). PNAS. 1-10).
Immunocytochemistry Analysis: A representative lot detected GRASP65 in NRK cells (Tang, D., et al. (2012). Biology Open. 1204-1214).
Immunocytochemistry Analysis: A representative lot detected GRASP65 in CHO cells transfected with WT APP and PS1 or APPswe/PS1ΔE9 mutants, cultured primary hippocampal neurons, and WT CHO cells (Joshi, G., et al. (2013). PNAS. 1-10).
Western Blotting Analysis: A representative lot detected GRASP65 in APPswe/PS1ΔE9 cells stably expressing GFP-tagged GRASP65 WT, cells stably expressing GFP-tagged GRASP65 WT, and mouse brain (Joshi, G., et al. (2013). PNAS. 1-10).
Biological Information
ImmunogenGST-tagged Recombinant protein corresponding to rat GRASP65.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Gorasp1
  • Grasp65
Purification MethodUnpurified
UniProt Number
Molecular Weight~65 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human alzheimer's brain tissue lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected GRASP65 in 10 µg of human alzheimer's brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
ABS1601 04055977161908