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MAB463 Anti-Estrogen Receptor Antibody, clone B10

MAB463
100 µL  
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Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RELISA, WB, IH(P)MAscitesMonoclonal Antibody
Description
Catalogue NumberMAB463
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Estrogen Receptor Antibody, clone B10
Alternate Names
  • ER
Product Information
FormatAscites
PresentationAscites. Liquid, does not contain any preservative.
Quality LevelMQ100
Applications
ApplicationUse Anti-Estrogen Receptor Antibody, clone B10 (Mouse Monoclonal Antibody) validated in ELISA, WB, IHC(P) to detect Estrogen Receptor also known as ER.
Key Applications
  • ELISA
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry (frozen sections, microwave oven treated paraffin sections) at 1:1,000-1:2,000

Immunocytochemistry (formaldehyde fixation) at 1:1,000-1:2,000

ELISA at 1:1,000-1:2,000

Western blot analysis at 1:1,000-1:2,000

Immunoprecipitation at 1:1-1:10

Gel super shifts at 1:1-1:10

Optimal working dilutions must be determined by the end user.
Biological Information
Epitopea.a. 151-165
CloneB10
HostMouse
SpecificityThe peptide used as the immunogen for this product correspond to the amino-acid sequence 151-165 from human ER alpha receptor. By IP, western, gel retardation and ELISA assays clone B10 do not react with other nuclear receptors.

SPECIES REACTIVITIES:

Does not cross react with chicken ER. Other reactivities have not been evaluated. By sequence homology pig ER is 100% identical, rat is 80% conserved.
IsotypeIgG2a
Species Reactivity
  • Human
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • HSPG2
  • SJA
  • perlecan
  • SJS
  • HSPG
  • PRCAN
  • Perlecan
  • SJS1
  • PLC
UniProt Number
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots up to 12 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size100 µL
Global Trade Item Number
Numer katalogowy GTIN
MAB463 04053252666230