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MABE160 Anti-E2F4 Antibody, clone LLF4-2

Anti-E2F4 Antibody, clone LLF4-2 is a Mouse Monoclonal Antibody for detection of E2F4 also known as Transcription factor E2F4, E2F-4 & has been validated in WB & IHC.

Anti-E2F4 Antibody, clone LLF4-2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: E2F4 UniProt Number: Q16254
MABE160
100 µg  
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Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE160
DescriptionAnti-E2F4 Antibody, clone LLF4-2
Alternate Names
  • Transcription factor E2F4
  • E2F-4
Background InformationE2F4 is the most common E2F family member in a number of cell types. E2F4 deficiency has been shown to cause a decrease in the thickness of the gut epithelium, decrease in maturation of hematopoietic cell types and an increased rate of apoptosis, suggesting that E2F4 plays a role in normal maturation of several cell types. E2F4 has also been shown to be essential for the normal development of airway epithelium and early eye development. Other data has implicated E2F4 during normal heart development, specifically cardiomyocyte proliferation.
References
Product Information
FormatPurified
Control
  • HeLa cell lysate
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-E2F4 Antibody, clone LLF4-2 is a Mouse Monoclonal Antibody for detection of E2F4 also known as Transcription factor E2F4, E2F-4 & has been validated in WB & IHC.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Kinross, K., et al. (2006). Blood. 108(3):886-895.)
Biological Information
ImmunogenHistidine-tagged recombinant protein corresponding to human E2F4.
CloneLLF4-2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer. [provided by RefSeq].
Gene Symbol
  • E2F4
Purification MethodProtein G
UniProt Number
UniProt SummaryFUNCTION: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.

SUBUNIT STRUCTURE: Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with DP-1 to E2F sites. The E2F4/DP-1 dimer interacts preferentially with pocket protein RBL1, which inhibits the E2F transactivation domain. Lower affinity interaction has been found with retinoblastoma protein RB1. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Interacts with HCFC1. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2.

SUBCELLULAR LOCATION: Nucleus.

TISSUE SPECIFICITY: Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

DEVELOPMENTAL STAGE: Present in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle.

PTM: Differentially phosphorylated in vivo.

POLYMORPHISM: The poly-Ser region of E2F4 is polymorphic and the number of Ser varies in the population (from 8 to 17). The variation might be associated with tumorigenesis.

SEQUENCE SIMILARITIES: Belongs to the E2F/DP family.
Molecular WeightBands may be detected at ~45 and ~60 kDA
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected E2F4 on 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
MABE160 04053252419607

Documentation

Anti-E2F4 Antibody, clone LLF4-2 MSDS

Title

Safety Data Sheet (SDS) 

Anti-E2F4 Antibody, clone LLF4-2 Certificates of Analysis

TitleLot Number
Anti-E2F4, clone LLF4-2 - 2424812 2424812
Anti-E2F4, clone LLF4-2 - 3216002 3216002
Anti-E2F4, clone LLF4-2 - 3313140 3313140
Anti-E2F4, clone LLF4-2 - 3819976 3819976
Anti-E2F4, clone LLF4-2 - 4108264 4108264
Anti-E2F4, clone LLF4-2 - NRG1897812 NRG1897812
Anti-E2F4, clone LLF4-2 -2567118 2567118
Anti-E2F4, clone LLF4-2 -2847008 2847008
Anti-E2F4, clone LLF4-2 -2857479 2857479
Anti-E2F4, clone LLF4-2 Monoclonal Antibody 3129260
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Brochure

Title
New Products: Volume 3, 2012