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324408 Anti-Dopamine D₅ Receptor Rabbit pAb

324408
Purchase on Sigma-Aldrich

Przegląd

Replacement Information

Tabela kluczowych gatunków

Species ReactivityHostAntibody Type
H, RRbPolyclonal Antibody

Products

Numer katalogowyOpakowanie Ilość/opak.
324408-100UL Ampulka plastikowa 100 ul
Description
OverviewRecognizes the dopamine D5 receptor in rat brain. Does not cross-react with other dopamine receptors.
Catalogue Number324408
Brand Family Calbiochem®
References
ReferencesMcKenna, F., et al. 2002. J. Neuroimmunol. 132, 34.
Product Information
FormLyophilized
FormulationUndiluted serum.
Positive controlRat brain
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesFlow Cytometry McKenna, F., et al. 2002. J. Neuroimmunol. 132, 34.
Key Applications Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Immunoblotting (Western Blotting)
Immunohistochemistry
Application NotesELISA (see comments)
Flow Cytometry (1:100, see application references)
Immunoblotting (1:200)
Immunohistochemistry (1:2500)
Application CommentsFor immunohistochemistry, a 1:2500 dilution of the reconstituted antiserum demonstrated staining of specific cells in various regions of rat brain sections, including Cortex layer 5 and parafascicular nucleus. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic peptide (LPPGSNGTAYC) corresponding to amino acids 2-10 of human dopamine D₅ receptor, conjugated to carrier protein
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
324408-100UL 04055977215878

Documentation

Anti-Dopamine D₅ Receptor Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

Anti-Dopamine D₅ Receptor Rabbit pAb Certificates of Analysis

TitleLot Number
324408

References

Przegląd literatury
McKenna, F., et al. 2002. J. Neuroimmunol. 132, 34.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-July-2007 RFH
ApplicationELISA (see comments)
Flow Cytometry (1:100, see application references)
Immunoblotting (1:200)
Immunohistochemistry (1:2500)
DescriptionRabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~49 kDa D5 dopamine receptor protein.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide (LPPGSNGTAYC) corresponding to amino acids 2-10 of human dopamine D₅ receptor, conjugated to carrier protein
IsotypeIgG
Specieshuman, rat
Positive controlRat brain
FormLyophilized
FormulationUndiluted serum.
PreservativeNone
CommentsFor immunohistochemistry, a 1:2500 dilution of the reconstituted antiserum demonstrated staining of specific cells in various regions of rat brain sections, including Cortex layer 5 and parafascicular nucleus. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).
Toxicity Standard Handling
ReferencesMcKenna, F., et al. 2002. J. Neuroimmunol. 132, 34.
Application referencesFlow Cytometry McKenna, F., et al. 2002. J. Neuroimmunol. 132, 34.