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ST1650 Anti-DYRK1A Mouse mAb (7D10)

Anti-DYRK1A, mouse monoclonal, clone 7D10, recognizes the ~80-85 kDa DYRK1A in PC-12, 293, HeLa NE, Raw 264.7, & NIH3T3 cells. It is validated for use in ELISA and Western blotting.

Anti-DYRK1A Mouse mAb (7D10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Dual-Specificity Tyrosine-(Y)-Phosphorylation Regulated Kinase 1A

ST1650
Purchase on Sigma-Aldrich

Przegląd

Replacement Information

Tabela kluczowych gatunków

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Products

Numer katalogowyOpakowanie Ilość/opak.
ST1650-100UG 100 μg
Description
OverviewRecognizes the ~80-85 kDa DYRK1A protein in PC-12, 293, HeLa NE, Raw 264.7, and NIH3T3 cells.
Catalogue NumberST1650
Brand Family Calbiochem®
SynonymsAnti-Dual-Specificity Tyrosine-(Y)-Phosphorylation Regulated Kinase 1A
References
ReferencesGuo, X., et al. 2010. J. Biol. Chem. 285, 13223.
Guedj, F., et al. 2009. PLoS One. 4, e4606.
Hamelet, J., et al. 2009. Biochem. Biophys. Res. Commun. 378, 673.
Lee, Y., et al. 2009. J. Biol. Chem. 284 33343.
Noll, C., et al. 2009. PLoS One 2009. 4, e7540.
Scales, T.M., et al. 2009. J. Cell Sci. 122, 2424.
Aranda, S., et al. 2008. Mol. Cell Biol. 28, 5899.
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlPC-12 cells, 293 cells, HeLa NE cells, Raw 264.7 cells, NIH3T3 cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids of 674-763 human DYRK1A, expressed as a GST fusion protein
Clone7D10
HostMouse
IsotypeIgG2b
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
ST1650-100UG 04055977223798

Documentation

Anti-DYRK1A Mouse mAb (7D10) MSDS

Title

Safety Data Sheet (SDS) 

References

Przegląd literatury
Guo, X., et al. 2010. J. Biol. Chem. 285, 13223.
Guedj, F., et al. 2009. PLoS One. 4, e4606.
Hamelet, J., et al. 2009. Biochem. Biophys. Res. Commun. 378, 673.
Lee, Y., et al. 2009. J. Biol. Chem. 284 33343.
Noll, C., et al. 2009. PLoS One 2009. 4, e7540.
Scales, T.M., et al. 2009. J. Cell Sci. 122, 2424.
Aranda, S., et al. 2008. Mol. Cell Biol. 28, 5899.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-November-2010 JLN
SynonymsAnti-Dual-Specificity Tyrosine-(Y)-Phosphorylation Regulated Kinase 1A
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
DescriptionPurified mouse monoclonal antibody. Recognizes the ~80-85 kDa DYRK1A protein.
BackgroundThis gene encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5' UTR or in the 3' coding region. These variants encode at least five different isoforms.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids of 674-763 human DYRK1A, expressed as a GST fusion protein
Clone7D10
IsotypeIgG2b
Specieshuman, mouse, rat
Positive controlPC-12 cells, 293 cells, HeLa NE cells, Raw 264.7 cells, NIH3T3 cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review
ReferencesGuo, X., et al. 2010. J. Biol. Chem. 285, 13223.
Guedj, F., et al. 2009. PLoS One. 4, e4606.
Hamelet, J., et al. 2009. Biochem. Biophys. Res. Commun. 378, 673.
Lee, Y., et al. 2009. J. Biol. Chem. 284 33343.
Noll, C., et al. 2009. PLoS One 2009. 4, e7540.
Scales, T.M., et al. 2009. J. Cell Sci. 122, 2424.
Aranda, S., et al. 2008. Mol. Cell Biol. 28, 5899.