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ABN66 Anti-Cholesterol acyltransferase 1 Antibody

This Anti-Cholesterol acyltransferase 1 Antibody is validated for use in WB, IH(P) for the detection of Cholesterol acyltransferase 1.

Anti-Cholesterol acyltransferase 1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurodegenerative Diseases Gene Symbol: SOAT1, ACACT, ACACT1, ACAT, ACAT1, SOAT, STAT UniProt Number: P35610
ABN66
100 µg  
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Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, Rhesus Macaque, Chp, RWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN66
DescriptionAnti-Cholesterol acyltransferase 1 Antibody
Alternate Names
  • Sterol O-acyltransferase 1
  • Acyl-coenzyme A:cholesterol acyltransferase 1
  • ACAT-1
  • Cholesterol acyltransferase 1
Background InformationCholesterol acyltransferase 1 (ACAT1) is mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA and plays a significant role in ketone body metabolism. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. Recent studies suggests that ACAT1 may be a therapeutic target for treating certain forms of Alzeheimer's disease.
References
Product Information
FormatAffinity Purified
Control
  • Caco-2 cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Cholesterol acyltransferase 1 Antibody is validated for use in WB, IH(P) for the detection of Cholesterol acyltransferase 1.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Cholesterol acyltransferase 1 in human adrenal gland and normal human brain tissues.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Cholesterol acyltransferase 1.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
  • Rhesus Macaque
  • Chimpanzee
  • Rat
Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Rhesus Macaque, Chimpanzee, and Rat based on 100% sequence homology. Other homologies: Mouse (93% sequence homology).
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryAcyl-coenzyme A:cholesterol acyltransferase (ACACT; EC 2.3.1.26) is an intracellular protein located in the endoplasmic reticulum that forms cholesterol esters from cholesterol. Accumulation of cholesterol esters as cytoplasmic lipid droplets within macrophages and smooth muscle cells is a characteristic feature of the early stages of atherosclerotic plaques (Cadigan et al., 1988).
Gene Symbol
  • SOAT1
  • ACACT
  • ACACT1
  • ACAT
  • ACAT1
  • SOAT
  • STAT
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Catalyzes the formation of fatty acid-cholesterol esters. Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase.

CATALYTIC ACTIVITY: Acyl-CoA + cholesterol = CoA + cholesterol ester.

SUBUNIT STRUCTURE: May form homo- or heterodimers.

SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein.

INDUCTION: Highly activated by the presence of cholesterol.

SEQUENCE SIMILARITIES: Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.
Molecular Weight~58 kDa observed which may be the result of dimerization/aggregation of the native protein during sample preparation for SDS-PAGE. The calculated molecular weight is 65 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in Caco-2 cell lysate.

Western Blot Analysis: 0.05 µg/mL of this antibody detected Cholesterol acyltransferase 1 on 10 µg of Caco-2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
ABN66 04053252592041

Documentation

Anti-Cholesterol acyltransferase 1 Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-Cholesterol acyltransferase 1 Antibody Certificates of Analysis

TitleLot Number
Anti-Cholesterol acyltransferase 1 - 3229433 3229433
Anti-Cholesterol acyltransferase 1 - 3386432 3386432
Anti-Cholesterol acyltransferase 1 - 3519822 3519822
Anti-Cholesterol acyltransferase 1 - 3638907 3638907
Anti-Cholesterol acyltransferase 1 - 3829458 3829458
Anti-Cholesterol acyltransferase 1 - NRG1920078 NRG1920078
Anti-Cholesterol acyltransferase 1 -2567123 2567123
Anti-Cholesterol acyltransferase 1 Polyclonal Antibody 3776145
Anti-Cholesterol acyltransferase 1 Polyclonal Antibody 2900036