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MABF770 Anti-CDK9, clone 1B5D10 Antibody

Detect CDK9 using this Anti-CDK9, clone 1B5D10 Antibody validated for use in Western Blotting, Immunohistochemistry and ELISA.

Anti-CDK9, clone 1B5D10 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Apoptosis - Additional Gene Symbol: CDK9, CDC2L4, TAK UniProt Number: P50750
MABF770
100 µL  
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Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHC, ELISAMAscitesMonoclonal Antibody
Description
Catalogue NumberMABF770
DescriptionAnti-CDK9, clone 1B5D10 Antibody
Alternate Names
  • Cyclin-dependent kinase 9
  • CDK9
Background InformationCDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. CDK9 forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.
References
Product Information
FormatAscites
PresentationMouse monoclonal IgG1 ascitic fluid containing 0.03% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect CDK9 using this Anti-CDK9, clone 1B5D10 Antibody validated for use in Western Blotting, Immunohistochemistry and ELISA.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • ELISA
Application NotesImmunohistochemistry Analysis (IHC): A 1:200-1,000 dilution from a representative lot detected CDK9 in endometrial cancer and rectum cancer tissues.

ELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Biological Information
ImmunogenPurified recombinant fragment of human CDK9 expressed in E. Coli. 
Clone1B5D10
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • CDK9
  • CDC2L4
  • TAK
Purification MethodUnpurified
UniProt Number
Molecular Weight~43 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Jurkat, A431 and HEK293 cell lysates.

Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected CDK9 in Jurkat, A431 and HEK293 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
MABF770 04055977171853