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05-1321 Anti-BMI1 Antibody, clone AF27

Anti-BMI1 Antibody, clone AF27 is a mouse monoclonal antibody for detection of BMI1 also known as BMI1 polycomb ring finger oncogene, murine leukemia viral (bmi-1) oncogene homolog & has been validated in IP, ChIP.

Anti-BMI1 Antibody, clone AF27 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: MGC12685, PCGF4, RNF51, flvi-2/bmi-1 UniProt Number: P35226
05-1321
100 µg  
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Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIP, ChIPMPurifiedMonoclonal Antibody
Description
Catalogue Number05-1321
DescriptionAnti-BMI1 Antibody, clone AF27
Alternate Names
  • B lymphoma Mo-MLV insertion region 1 homolog
  • B lymphoma Mo-MLV insertion region 1 homolog (mouse)
  • BMI1 polycomb ring finger oncogene
  • Polycomb group RING finger protein 4
  • RING finger protein 51
  • murine leukemia viral (bmi-1) oncogene homolog
  • oncogene BMI-1
  • polycomb group protein Bmi1
  • polycomb group ring finger 4
Background InformationThe BMI1 protein is a member of the polycomb group (PcG) proteins. PcG proteins form multiprotein complexes that function as transcriptional repressors. Several lines of evidence implicate BMI1 in tumorigenesis. BMI1 cooperates with c-Myc in the generation of lymphomas in double transgenic mice. BMI1 blocks senescence and immortalizes mouse embryo fibroblasts, although not human fibroblasts, and in combination with an activated H-ras gene, leads to neoplastic transformation. The BMI1 gene is amplified in certain mantle cell lymphomas and is overexpressed in a subset of non-small cell lung cancer, colorectal carcinomas, multiple myelomas and medulloblastomas, but not glioblastomas. The transformation of human hematopoietic progenitor cells by the oncogenic fusion protein E2a-Pbx1 requires BMI1.
References
Product Information
FormatPurified
Control
  • MEF1 cell lysate
PresentationPurified mouse monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-BMI1 Antibody, clone AF27 is a mouse monoclonal antibody for detection of BMI1 also known as BMI1 polycomb ring finger oncogene, murine leukemia viral (bmi-1) oncogene homolog & has been validated in IP, ChIP.
Key Applications
  • Immunoprecipitation
  • Chromatin Immunoprecipitation (ChIP)
Application NotesChromatin Immunoprecipitation (ChIP):
A previous lot was used by an independent laboratory in ChIP. (Bracken, A.P., et al., 2007).
Biological Information
ImmunogenMBP-tagged recombinant protein corres-ponding to full length human BMI1.
CloneAF27
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes BMI1.
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MGC12685
  • PCGF4
  • RNF51
  • flvi-2/bmi-1
Purification MethodAnti-BMI1, clone AF27
UniProt Number
UniProt SummaryFUNCTION: Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
SUBUNIT STRUCTURE: Component of chromatin-associated class II polycomb repressive complex 1 (PRC1/hPRC-H) at least composed of PCGF2/RNF110, BMI1/PCGF4, CBX2/M33, CBX4/PC2, CBX8/PC3, PHC1, PHC2, PHC3, SCMH1, RING1 and RNF2/RING2. Also component of E3 ubiquitin ligase complex or human polycomb repressive complex 1-like at least composed of PHC2, BMI1, RING1 and RNF2. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm.
PTM: May be polyubiquitinated; which does not lead to proteasomal degradation.
INVOLVEMENT IN DISEASE: Cooperates with the MYC oncogene to produce B-lymphomas.
SEQUENCE SIMILARITIES: Contains 1 RING-type zinc finger.
Molecular Weight~35 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunoprecipitation in MEF1 cell lysate.
Immunoprecipitation Analysis: 10 μg/mL of this antibody immunoprecipitated BMI1 from 100 μg of MEF1 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable at 2-8°C for 1 year from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
05-1321 04053252408649

Documentation

Anti-BMI1 Antibody, clone AF27 MSDS

Title

Safety Data Sheet (SDS) 

Anti-BMI1 Antibody, clone AF27 Certificates of Analysis

TitleLot Number
Anti-BMI1, clone AF27 3082033
Anti-BMI1, clone AF27 - 2430415 2430415
Anti-BMI1, clone AF27 - 1941606 1941606
Anti-BMI1, clone AF27 - 2023198 2023198
Anti-BMI1, clone AF27 - 2262738 2262738
Anti-BMI1, clone AF27 - NG1681858 NG1681858
Anti-BMI1, clone AF27 - NG1740996 NG1740996
Anti-BMI1, clone AF27 - NG1829872 NG1829872
Anti-BMI1, clone AF27 - NG1853779 NG1853779
Anti-BMI1, clone AF27 - NRG16443470 NRG16443470
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