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04-771 Anti-Akt2/PKBβ Antibody, clone AW114, rabbit monoclonal

Anti-Akt2/PKBβ Antibody, clone AW114 detects level of Akt2/PKBβ & has been published & validated for use in WB & IP.

Anti-Akt2/PKBβ Antibody, clone AW114, rabbit monoclonal MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: PI3K, Akt, & mTOR Signaling Gene Symbol: EC 2.7.11.1, PKBB, PKBBETA, PRKBB , RAC-BETA, RAC-PK-beta UniProt Number: P31751
04-771
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Tabela kluczowych gatunków

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, IPRbCulture SupernatantMonoclonal Antibody
Description
Catalogue Number04-771
Replaces04-406
DescriptionAnti-Akt2/PKBβ Antibody, clone AW114, rabbit monoclonal
Alternate Names
  • AKT2 kinase
  • Murine thymoma viral (v-akt) homolog-2
  • PKB beta
  • Protein kinase Akt-2
  • Protein kinase B, beta
  • rac protein kinase beta
  • v-akt murine thymoma viral oncogene homolog 2
Background InformationAkt is the major known effector of the PI3 Kinase pathway. Generation of PIP3 results in the activation of PDK1, which phosphorylates Akt on Thr308, and another kinase complex, thought to be the mTORC2 complex (or possibly an intermediate kinase linking the two) that phosphorylates Akt on Ser473. These phosphorylations additively activate Akt Ser/Thr kinase activity, and the use of phosphorylation state-specific antibodies directed against either of these sites can imply Akt activation. Activation of Akt can be measured directly by immunoprecipitation followed by phosphorylation of a known substrate with radiolabeled ATP. Akt has been shown to phosphorylate over 70 substrates including TSC1&2 in the mTOR pathway, Bad on Ser136, GSK3 (which is inactivated by this phosphorylation), and the FoxO family of transcription factors (PRAS40, AS160).
References
Product Information
FormatCulture Supernatant
Control
  • L6 cell lysate
PresentationCultured supernantant containing 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Akt2/PKBβ Antibody, clone AW114 detects level of Akt2/PKBβ & has been published & validated for use in WB & IP.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Biological Information
ImmunogenKLH-conjugated, synthetic peptide (CRYDSLGSLELDQRTH) corresponding to amino acids 455-469 of human Akt2/PKBβ, with an N-terminal cysteine added for conjugation purposes.
Epitopea.a. 455-469
CloneAW114
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes Akt2/PKBβ, Mr ~60 kDa.
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteHuman and rat. Predicted to cross-react with mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins.
Gene Symbol
  • EC 2.7.11.1
  • PKBB
  • PKBBETA
  • PRKBB
  • RAC-BETA
  • RAC-PK-beta
UniProt Number
UniProt SummaryFUNCTION: General protein kinase capable of phosphorylating several known proteins.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
ENZYME REGULATION:Two specific sites, one in the kinase domain (Thr-309) and the other in the C-terminal regulatory region (Ser-474), need to be phosphorylated for its full activation.
SUBUNIT STRUCTURE: Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B.
TISSUE SPECIFICITY:In all human cell types so far analyzed.
INVOLVEMENT IN DISEASE: Alterations of AKT2 may contribute to the pathogenesis of ovarian carcinomas.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Molecular Weight~60 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceWestern Blot Analysis: A 1:500 dilution of this antibody detected Akt2/PKBβ in HepG2 lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µl
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
04-771 04053252589157