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ST1558 Anti-ALOX12 Mouse mAb (2D10)

Anti-ALOX12, mouse monoclonal, clone 2D10, recognizes the ALOX12 protein. It is validated for ELISA and Western blotting.

Anti-ALOX12 Mouse mAb (2D10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Arachidonate 12-Lipoxygenase

ST1558
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Przegląd

Replacement Information

Tabela kluczowych gatunków

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Numer katalogowyOpakowanie Ilość/opak.
ST1558-100UG 100 μg
Description
OverviewRecognizes the ALOX12 protein.
Catalogue NumberST1558
Brand Family Calbiochem®
SynonymsAnti-Arachidonate 12-Lipoxygenase
Application Data
Detection of human ALOX12 by immunoblotting. Sample: Partial, recombinant, human ALOX12 protein. Primary antibody: Anti-ALOX12 Mouse mAb (2D10) (Cat. No. ST1558) (1-5 µg/ml). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids 564-664 of human ALOX12, expressed as a GST fusion protein
Clone2D10
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
ST1558-100UG 04055977224283

Documentation

Anti-ALOX12 Mouse mAb (2D10) MSDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-November-2010 RFH
SynonymsAnti-Arachidonate 12-Lipoxygenase
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human ALOX12 by immunoblotting. Sample: Partial, recombinant, human ALOX12 protein. Primary antibody: Anti-ALOX12 Mouse mAb (2D10) (Cat. No. ST1558) (1-5 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ALOX12 protein.
BackgroundLipoxygenases (LOXs) are a family of non-heme iron dioxygenases that are involved in the production and metabolism of fatty acid hydroperoxidases. There are six lipoxygenase isozymes; 5-LOX, 12R-LOX, 12S-LOX, 15-LOX-1 and 15-LOX-2, which catalyze the regio-selective addition of oxygen into arachidonic acid, and epidermal LOX (E-LOX) which metabolizes 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE) to a specific epoxyalcohol compound. Different isozymes have different physiological roles; 5-, 12R-, 12S- and 15-LOX-1 have proliferative, antiapoptotic and angiogenic functions whilst 15-LOX-2 has roles in differentiation, growth arrest and the induction of apoptosis. E-LOX, the most divergent member of this family, is involved in terminal differentiation of keratinocytes and has a major role in skin barrier function. Perturbations in lipoxygenase enzymes have been implicated in various human diseases including bronchial asthma, atherosclerosis, osteoporosis and some cancers.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids 564-664 of human ALOX12, expressed as a GST fusion protein
Clone2D10
IsotypeIgG2a
Specieshuman
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review