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MABF235 Anti-AIRE Antibody, clone Mab155

MABF235
100 µg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, WB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF235
DescriptionAnti-AIRE Antibody, clone Mab155
Alternate Names
  • Autoimmune regulator
  • Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein
  • APECED protein
Background InformationAIRE (Autoimmune Regulator) is a human gene that is expressed primarily in the thymus. It causes transcription of a wide selection of organ-specific genes, reducing the threat of autoimmunity occurring by allowing the elimination of autoreactive T cells via negative selection. Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendo-crinopathy-candidiasis-ectodermal dystrophy (APECED). Disruption of AIRE results in the development of a range of autoimmune diseases, the most common clinical conditions in the syndrome are hypoparathyroidism, primary adrenocortical failure and chronic mucocutaneous candidiasis. Aire functions through initiating the transcription of a diverse set of self-antigens, such as insulin, in the thymus. This expression then allows maturing thymocytes to become tolerant towards peripheral organs, thereby suppressing autoimmune disease. Aire is atranscriptional regulator protein that binds to DNA as dimer and tetramer, but not as a monomer. It forms nuclear bodies and interacts with the transcriptional coactivator CBP (CREBBP). At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-AIRE Antibody, clone Mab155 is validated for use in Flow Cytometry and Western Blotting and Immunocytochemistry for the detection of AIRE.
Key Applications
  • Flow Cytometry
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: A representative lot detected AIRE in Cos1 transfected cell lysate (Rinderle, C., et al. (1999). Human Molecular Genetics. 8(2):277-290).
Immunocytochemistry Analysis: A representative lot detected AIRE in freshly isolated endogenous peripheral blood lymphocytes (Rinderle, C., et al. (1999). Human Molecular Genetics. 8(2):277-290).
Biological Information
ImmunogenHis-tagged recombinant protein corresponding to human AIRE.
CloneMab155
Concentration1 mg/mL
HostMouse
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • AIRE
  • APECED
Purification MethodProtein G Purified
UniProt Number
Molecular Weight58 kDa calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in human PBMC cells.

Flow Cytometry Analysis: 0.5 µg of this antibody detected AIRE in human PBMC cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
MABF235 04053252982644

Documentation

Anti-AIRE Antibody, clone Mab155 MSDS

Title

Safety Data Sheet (SDS) 

Anti-AIRE Antibody, clone Mab155 Certificates of Analysis

TitleLot Number
Anti-AIRE, clone Mab155 -3094692 3094692
Anti-AIRE, clone Mab155 - Q2438613 Q2438613
Anti-AIRE, clone Mab155 - 3175956 3175956

References

Reference overviewPub Med ID
AIRE encodes a nuclear protein co-localizing with cytoskeletal filaments: altered sub-cellular distribution of mutants lacking the PHD zinc fingers.
Rinderle, C, et al.
Hum. Mol. Genet., 8: 277-90 (1999)  1998

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