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14-555M Alk Protein, active, 250 µg

Active, N-terminal His6-tagged, recombinant human ALK, residues 1058-end, for use in Kinase Assays.

Alk Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-555M
250 µg  Also available in 10 μg size (cat#14-555) and in bulk (cat# 14-555-K).
Purchase on Sigma-Aldrich

Przegląd

Replacement Information
Description
Catalogue Number14-555M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAlk Protein, active, 250 µg
OverviewN-terminal His6-tagged, recombinant human ALK, residues 1058-end
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, N-terminal His6-tagged, recombinant human ALK, residues 1058-end, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed by baculovirus in Sf21 cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThe 2;5 chromosomal translocation is frequently associated with anaplastic large cell lymphomas (ALCLs). The translocation creates a fusion gene consisting of the ALK (anaplastic lymphoma kinase) gene and the nucleophosmin (NPM) gene: the 3' half of ALK, derived from chromosome 2, is fused to the 5' portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. These sequences are absent in the product of the transforming NPM-ALK gene. ALK shows the greatest sequence similarity to LTK (leukocyte tyrosine kinase). ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system.
Gene Symbol
  • ALK
  • CD246
  • TFG/ALK
Protein TargetALK
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyTK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9UM73 # Orphan receptor with a tyrosine-protein kinase activity. Appears to play an important role in the normal development and function of the nervous system. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif.
SIZE: 1620 amino acids; 176428 Da
SUBUNIT: Homodimer. When bound to ligand.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells.
PTM: N-glycosylated.
DISEASE: SwissProt: Q9UM73 # A chromosomal aberration involving ALK is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with NPM1. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated. The constitutively active fusion proteins are responsible for 5-10% of non-Hodgkin lymphomas. & A chromosomal aberration involving ALK is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15) with CARS; translocation t(2;4)(p23;q21) with SEC31A. & A chromosomal aberration involving ALK is associated with anaplastic large-cell lymphoma (ALCL). Translocation t(2;17)(p23;q25) with ALO17.
SIMILARITY: SwissProt: Q9UM73 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. & Contains 1 LDL-receptor class A domain. & Contains 2 MAM domains. & Contains 1 protein kinase domain.
Molecular Weight63.8kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of IGF-1Rtide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C from date of shipment
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-555) and in bulk (cat# 14-555-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Numer katalogowy GTIN
14-555M 04053252378669

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Numer katalogowy Opis
14-555 Alk Protein, active, 10 µg

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Kategorie

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases