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70954 Rosetta™(DE3) Competent Cells - Novagen

Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Rosetta™(DE3) Competent Cells - Novagen: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
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70954
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70954-4
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      Description
      OverviewRosetta™ host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for AGG, AGA, AUA, CUA, CCC, GGA codons on a compatible chloramphenicol-resistant plasmid. Thus the Rosetta strains provide for “universal” translation which is otherwise limited by the codon usage of E. coli. The tRNA genes are driven by their native promoters. In Rosetta(DE3)pLysS, the rare tRNA genes are present on the same plasmids that carries the T7 lysozyme gene.

      DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
      Genotype: F- ompT hsdSB(rB- mB-) gal dcm (DE3) pRARE (CamR)


      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.




      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      Catalogue Number70954
      Brand Family Novagen®
      References
      Product Information
      Components
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      70954-4 07790788052911

      Documentation

      Rosetta™(DE3) Competent Cells - Novagen Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Rosetta™(DE3) Competent Cells - Novagen Certificados de análisis

      CargoNúmero de lote
      70954

      Folleto

      Cargo
      Competent Cell Brochure

      Citas

      Título
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    • Anette Thysse Jonstrup, et al. (2007) The 1.4-Å crystal structure of the S. pombe Pop2p deadenylase subunit unveils the configuration of an active enzyme. Nucleic Acids Research 35, 3153-3164.
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    • Changkeun Lee, et al. (2007) Yeast mitochondrial initiator tRNA is methylated at G37 by the TRM5-encoded tRNA (Guanine-N1-)-methyltransferase. Journal of Biological Chemistry 282, 27744-27753.
    • Veli-Matti Leppänen, et al. (2007) Crystal structure of the N-terminal NC4 domain of collagen IX, a zinc binding member of the laminin-neurexin-sex hormone binding globulin (LNS) domain family. Journal of Biological Chemistry 282, 23219-23230.
    • Yoshihiro Nakamura, et al. (2007) Crystal structure of the human BRD2 bromodomain: insights into dimerization and recognition of acetylated histone H4. journal of Biological Chemistry 282, 4193-4201.
    • Pascale Plamondon, Nicole R. Luke and Anthony A. Campagnari. (2007) Identification of a novel two-partner secretion locus in Moraxella catarrhalis. Infection and Immunity 75, 2929-2936.
    • Biagio Pucci, et al. (2007) Modular organization of the Sulfolobus solfataricus mini-chromosome maintenance protein. Journal of Biological Chemistry 282, 12574-12582.
    • Giuseppe D. Tocchini-Valentini, Paolo Fruscoloni and Glauco P. Tocchini-Valentini. (2007) The dawn of dominance by the mature domain in tRNA splicing. Procedings of the National Academy of Science 104, 12300-12305.
    • Marielle E. Yohe, et al. (2007) Auto-inhibition of the Dbl family protein Tim by an N-terminal helical motif. Journal of Biological Chemistry 282, 13813-13823.
    • Zubair M. Ahmed, et al. (2006) The tip-link antigen, a protein associated with the transduction complex of sensory hair cells, is protocadherin-15. Journal of Neuroscience 26, 7022-7034.
    • Kenji Kashiwagi, et al. (2006) Frame shuffling: a novel method for in vitro protein evolution. Protein Engineering Design and Selection 19, 135-140.
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    • Chad E. Metcalf and David A. Wassarman. (2006) DNA binding properties of TAF1 isoforms with two AT-hooks. Journal of Biological Chemistry 281, 30015-30023.
    • James Schelert, et al. (2006) Regulation of mercury resistance in the crenarchaeote Sulfolobus solfataricus. Journal of Bacteriology 188, 7141-7150.
    • Yi-Hsien Su and Victor D. Vacquier. (2006) Cyclic GMP-specific phosphodiesterase-5 regulates motility of sea urchin spermatozoa. Molecular Biology of the Cell 17, 114-121.
    • Guido Veit, et al. (2006) Collagen XXVIII, a novel von Willebrand Factor A domain-containing protein with many imperfections in the collagenous domain. Journal of Biological Chemistry 281, 3494-3504.
    • Jerome Vicogne, et al. (2006) Asymmetric phospholipid distribution drives in vitro reconstituted SNARE-dependent membrane fusion. Procedings of the National Academy of Science 103, 14761-14766.
    • Hao Xu and William Wickner. (2006) BEM1P is a positive regulator of the homotypic fusion of yeast vacuoles. Journal of Biological Chemistry 281, 27158-27166.
    • Philipp Beinker, et al. (2005) Biochemical coupling of the two nucleotide binding domains of ClpB: Covalent linkage is not a prerequisite for chaperone activity. Journal of Biological Chemistry 280, 37965-37973.
    • Eric R. Bonner, et al. (2005) Molecular basis of cysteine biosynthesis in plants: structural and functional analysis of O-acetylserine sulfhydrylase from Arabidopsis thaliana. Journal of Biological Chemistry 280, 38803-38813.
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    • Thomas T. Egelhoff, Daniel Croft and Paul A. Steimle. (2005) Actin activation of myosin heavy chain kinase A in Dictyostelium: a biochemical mechanism for the spatial regultion of myosin II filament disassembly. Journal of Biological Chemistry 280, 2879-2887.
    • Pierre Fotso, et al. (2005) Cog1p plays a central role in the organization of the yeast conserved oligomeric golgi (COG) complex. Journal of Biological Chemistry 280, 27613-27623.
    • Johannes Haugstetter, Thomas Blicher and Lars Ellgaard. (2005) Identification and characterization of a novel thioredoxin-related transmembrane protein of the endoplasmic reticulum. Journal of Biological Chemistry 280, 8371-8380.
    • Meike Hoffmeister, et al. (2005) Mitochondrial trans-2-enoyl-coA reductase of wax ester fermentation from Euglena gracilis defines a new family of enzymes involved in lipid synthesis. Journal of Biological Chemistry 280, 4329-4338.
    • Shinpei Katou, et al. (2005) Catalytic activation of plant MAPK phosphatase NtMKP1 by its physiological substrate SIPK, but not by calmodulins. Journal of Biological Chemistry 280, 39569-39581.
    • Christopher K. Kennaway, et al. (2005) Dodecameric structure of the small heat shock protein Acr1 from Mycobacterium tuberculosis. Journal of Biological Chemistry 280, 33419-33425.
    • Beth T. Vander Kooi, et al. (2005) The glucose-6-phosphatase catalytic subunit gene promoter contains both positive and negative glucocorticoid response elements. Molecular Endocrinology 19, 3001-3022.
    • Michael Kothe, et al. (2005) Direct demonstration of carbamoyl phosphate formation on the C-terminal domain of carbamoyl phosphate synthetase. Protein Science 14, 37-44.
    • Sabbi Lall, Fabio Piano and Richard E. Davis. (2005) Caenorhabditis elegans decapping proteins: localization and functional analysis of Dcp1, Dcp2, and DcpS during embryogenesis. Molecular Biology of the Cell 16, 5880-5890.
    • Peter A. Lemaire, Jeffrey Lary and James L. Cole. (2005) Mechanism of PKR activation: dimerization and kinase activation in the absence of double-stranded RNA. Journal of Molecular Biology 345, 81-90.
    • Roberta Leonardi, et al. (2005) A pantothenate kinase from Staphylococcus aureus refractory to feedback regulation by coenzyme A. Journal of Biological Chemistry
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      Protocolos de usuario

      Cargo
      TB009 Competent Cells
      TB053 Academic and Non-profit Laboratory Assurance Letter

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      Número de referencia Descripción  
      70956 Rosetta™(DE3)pLysS Competent Cells - Novagen Precios y disponibilidad

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      Rosetta™

      Categorías

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Competent Cells for Expression