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AP112P Goat Anti-Human IgG Antibody, HRP conjugate

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AP112P
2 mL  
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      Descripción

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      Description
      Catalogue NumberAP112P
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionGoat Anti-Human IgG Antibody, HRP conjugate
      OverviewGoat anti-Human IgG
      Background InformationIgG is a monomeric immunoglobulin, consists of two heavy chains (gamma) and two light chains. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin. It is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, providing protection to the fetus in its first weeks of life before its own immune system has developed. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
      Product Information
      FormatHRP
      PresentationPurified by immunoaffinity chromatography using antigen coupled to agarose beads. Lyophilized. Buffer is 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA. Contains no preservative.
      Quality LevelMQ100
      Applications
      ApplicationDetect Human IgG using this Goat anti-Human IgG Antibody, HRP conjugate validated for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesEIA: 1:5,000-1:100,000.
      Western blots: 1:5,000-1:200,000.
      Immunohistochemistry: 1:500-1:5,000.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenPrepared from purified human IgG.
      HostGoat
      SpecificityThe antibody reacts with the heavy chains of human IgG and with the light chains common to most human immunoglobulins.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Purification MethodImmunoAffinity Purified
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized product at 2°-8°C for up to 12 months from date of receipt. After reconstitution the product is stable for six weeks at 2°-8°C as an undiluted liquid. Reconstitute vial with 2 mL of distilled water. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of antibody (and buffer salts) will decrease to one-half of the original after the addition of glycerol and final working dilutions must be adjusted accordingly. Avoid repeated freeze thaw cycles.

      WARNING:
      Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
      Packaging Information
      Material Size2 mL
      Global Trade Item Number
      Número de referencia GTIN
      AP112P 08436037123030