chromatin+assay
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17-295 Sigma-AldrichChromatin Immunoprecipitation (ChIP) Assay Kit
Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using inexpensive protein A agarose beads.
Histone H4 Antibodies and Proteins
Millipore’s Histone H4 antibodies demonstrate specificity against linker histone H4. See below for H4 Antibodies, ChipAb+ Primer sets, and peptides, based on the expertise of Upstate & Chemicon.
Histone H1 Antibodies and Proteins
Millipore’s Histone H1 antibodies demonstrate specificity against linker histone H1. See below for pan- and phospho-H1 Antibodies and Proteins, and more, based on the expertise of Upstate & Chemicon.
16-157 Sigma-AldrichProtein A Agarose/Salmon Sperm DNA, 2.5 mL
Recombinant protein A covalently bound to agarose beads for use in chromatin immunoprecipitations (ChIP assays). Sonicated salmon sperm DNA is included to block non-specific DNA binding sites on agarose beads. Every lot qualified using an Acetyl Histone H4 and H3 ChIP assay.
16-201 Sigma-AldrichProtein G Agarose/Salmon Sperm DNA, 2.5 mL
Recombinant Protein G covalently bound to agarose beads by cyanogen bromide linkage for use in chromatin immunoprecipitations (ChIP assays). Sonicated salmon sperm DNA is included to block non-specific DNA binding sites on agarose beads. Every lot qualified using an Acetyl Histone H3 ChIP assay.
07-450 Sigma-AldrichAnti-monomethyl-Histone H3 (Lys9) Antibody
Anti-monomethyl-Histone H3 (Lys9) Antibody is a Rabbit Polyclonal Antibody for detection of Histone H3 monomethylated on lysine 9 (H3K9me1). This purified antibody is specificity verified by dot blot (DB), published in peer reviewed journals, and has been validated in ICC, PIA & WB.
13-107 Sigma-AldrichCore Histones
Highly pure core histones proteins (H2A, H2B, H3 and H4) including H1 purified from chicken erythrocytes, suitable as a substrate for histone modification assays (HAT, HDAC, DNMT) and nucleosome assembly studies.