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AC146 Normal Chicken IgY Antibody, purified | AC146

Normal Chicken IgY Antibody, purified | AC146 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Secondary & Control Antibodies Research Sub-Category: Isotype Control Antibodies
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AC146
100 µg  
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      Description
      Catalogue NumberAC146
      Trade Name
      • Chemicon
      DescriptionNormal Chicken IgY Antibody, purified | AC146
      Background InformationChickens transfer high quantities of IgY (IgG) into the egg yolk and harvesting antibodies from eggs eliminates the need for the invasive bleeding procedure. One week's eggs can contain 10 times more antibodies than the volume of rabbit blood obtained from one weekly bleeding. However, there are some disadvantages when using certain chicken derived antibodies in immunoassays. Chicken IgY does not fix mammalian complement component C1 and it does not perform as a precipitating antibody using standard solutions.
      Product Information
      FormatPurified
      PresentationPurified chicken polyclonal in buffer containing PBS with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      Key Applications
      • Blocking of Intefering Antibodies
      Application NotesGel Electrophoresis Analysis: 20 µg of reduced Chicken IgY from a representative lot was resolved by SDS-PAGE and visualized using total protein stain.

      Note: To be used as a negative control for ChIP Ab+ MeCP2, Cat. No. 17-10491.

      Note: For use as a negative control, perform experiments in parallel using primary antibody or normal serum at the same concentration. A signal obtained with the normal serum indicates a nonspecific interaction; any additional signal with the primary antibody indicates a specific antibody-antigen interaction.
      Biological Information
      Concentration1 mg/mL
      HostChicken
      IsotypeIgY
      Antibody TypePolyclonal Antibody
      Purification MethodT-gel
      Molecular Weight~180 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting using non-reduced Chicken IgY.

      Western Blotting Analysis: 0.01 µg of this non-reduced Chicken IgY was detected by a Rabbit anti-Chicken secondary antibody.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      AC146 04053252944727