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109261 Lugol's solution (diluted iodine-potassium iodide solution)

for the Gram staining method

Lugol's solution (diluted iodine-potassium iodide solution) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Iodine-potassium iodide solution

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109261
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      Description
      Catalogue Number109261
      SynonymsIodine-potassium iodide solution
      DescriptionLugol's solution (diluted iodine-potassium iodide solution)
      OverviewLugol’s solution (diluted iodine-potassium iodide solution - for the Gram staining method, is a ready-to-use solution used in Bacteriology, for example, for the staining of Gram bacteria. In bacteriology, the Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria wall is the basis of the color affinity. In the first step, bacteria will be stained with crystal violet (Cat.no. 109218), an aniline dye. After the treatment with iodine solution (Lugol’s solution), a dye-iodine complex will form. During the decolorizing step (Gram's decolorizing solution, Cat. no. 110218), this complex stays in the multilayer mureine structure of the cell wall of Gram-positive bacteria - they will appear blue-violet. Gram-negative bacteria, by contrast, have a cell wall consisting of a single-layered murein structure, and correspondingly re-release the staining dye with the decoloring solution. Gram-negative bacteria will be counterstained with safranine solution (Cat.no. 109217) and will then appear pink to red.
      Lugol's solution (diluted iodine-potassium iodide solution) is capable of staining up to 500 slides. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
      Product Information
      HS Code3212 90 00
      Quality LevelMQ400
      Applications
      Applicationfor the Gram staining method
      Physicochemical Information
      Boiling point100 °C (1013 hPa)
      Density1.01 g/cm3 (20 °C)
      pH value3.5 (H₂O, 20 °C)
      Safety Information according to GHS
      Storage class10 - 13 Other liquids and solids
      WGKWGK 2 obviously hazardous to water
      Storage and Shipping Information
      StorageStore at +15°C to +25°C.
      Specifications
      Suitability for microscopypasses test
      gram-positve microorganismsblue-violet
      gram-negative microorganismspink to red
      Global Trade Item Number
      Catalogue Number GTIN
      1092611000 04022536112701