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101287 Löffler's methylene blue solution

for microscopy

Löffler's methylene blue solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Löfflere'smethylene blue solution, Methylene blue solution acc. to Löffler

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101287
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      		 Plastic bottle 100 ml
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      1012872500
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          		 Plastic bottle 2.5 l
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          Description
          Catalogue Number101287
          SynonymsLöfflere'smethylene blue solution, Methylene blue solution acc. to Löffler
          DescriptionLöffler's methylene blue solution
          OverviewLöffler’s methylene blue solution - for microscopy, is a ready-to-use-solution and is used for human-medical cell diagnosis and serves the purpose of the bacteriological and histological investigation of sample material of human origin, for example smears of enriched bacterial cultures or histological sections of e. g. the lung. The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycolate-fuchsin complex in the cell wall. Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use methylene blue is used for counterstaining.
          Pretreatment of the specimens with Sputofluol® dissolves the bacteria from the surrounding viscid sputum and cell material.
          The 500 ml bottle is sufficient for approx. 250 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
          References
          Product Information
          HS Code3212 90 90
          Quality LevelMQ400
          Applications
          Applicationfor microscopy
          Biological Information
          Physicochemical Information
          Density0.97 g/cm3 (20 °C)
          Flash point40 °C
          pH value7.0 - 7.5 (H₂O, 20 °C)
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Hazard Pictogram(s) Hazard Pictogram(s)
          Hazard Statement(s)H226: Flammable liquid and vapour.
          Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
          P233: Keep container tightly closed.
          P240: Ground and bond container and receiving equipment.
          P241: Use explosion-proof electrical/ ventilating/ lighting equipment.
          P242: Use non-sparking tools.
          P243: Take action to prevent static discharges.
          Signal WordWarning
          Storage class3 Flammable liquids
          WGKWGK 1 slightly hazardous to water
          Disposal3
          Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
          Safety Information
          Categories of dangerflammable
          Product Usage Statements
          Storage and Shipping Information
          StorageStore at +15°C to +25°C.
          Packaging Information
          Transport Information
          Declaration (railroad and road) ADR, RIDUN 1993 , 3, III
          Declaration (transport by air) IATA-DGRUN 1993 , 3, III
          Declaration (transport by sea) IMDG-CodeUN 1993 , 3, III
          Supplemental Information
          Specifications
          Global Trade Item Number
          Catalogue Number GTIN
          1012870100 04022536019024
          1012872500 04022536019062

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