Millipore Sigma Vibrant Logo

400051 Hygromycin B, Streptomyces sp. - CAS 31282-04-9 - Calbiochem

This product can no longer be ordered through emdmillipore.com. Please refer to (Cat. No. 400051) on sigmaaldrich.com.

Hygromycin B, Streptomyces sp. - CAS 31282-04-9 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Primary Target: growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells
View Products on Sigmaaldrich.com
400051
View Pricing & Availability

Overview

Key Spec Table

CAS #Empirical Formula
31282-04-9C₂₀H₃₇N₃O₁₃

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
400051-1MU
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      		 Alu drum 1 mu
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      —
      Request Pricing
      		 —
      Add To Favorites
      400051-5MU
      —
      Limited Availability Limited Availability
      In Stock 
      Discontinued
      Limited Quantities Available
      Availability to be confirmed
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          		 Alu drum 5 mu
          Price could not be retrieved
          Minimum Quantity is a multiple of
          Maximum Quantity is
          Upon Order Completion More Information
          You Saved ()
           
          —
          Request Pricing
          		 —
          Add To Favorites
          Description
          OverviewUnique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells. Inhibits protein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA. Hph, a gene from E. coli, encodes resistance to hygromycin B and can be isolated and cloned by recombinant DNA techniques. This hygromycin B-resistance gene is particularly useful for identification or selection of recombinant clones in a variety of cell types. Hygromycin B penetrates cells that have been permeabilized by virus infection and can act as an effective antiviral agent.
          Bioassay: 350-450 units/mg Hygromycin B. 106 units is equivalent to ~900 mg.
          Supplied with a directional insert. Note: 1 KU = 1000 units; 1 MU = 1,000,000 units.
          Catalogue Number400051
          Brand Family Calbiochem®
          References
          ReferencesDean, N. 1995. Proc. Natl. Acad. Sci. USA 92, 1287.
          Hamada, W., et al. 1994. Curr. Genetics 26, 251.
          Hubbard, S. C., et al. 1994. J. Biol. Chem. 269, 3717.
          Rikkerink, E. H., et al. 1994. Current Genetics 25, 202.
          Sugimoto, K., et al. 1994. Plant J. 5, 863.
          Buchschacher, G.L., Jr., and Panganiban, A.T. 1992. J. Virol. 66, 2731.
          Gaken, J., et al. 1992. Biotechniques 13, 32.
          Lama, J., and Carrasco, L. 1992. J. Biol. Chem. 267, 15932.
          Ma, H., et al. 1992. Gene 117, 161.
          MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol. 276, 67.
          Norman, J.A., et al. 1992. Mol. Pharmacol. 41, 53.
          Bilang, R., et al. 1991. Gene 100, 247.
          Dale, E., and Ow, D. 1991. Proc. Natl. Acad. Sci. USA 88, 10558.
          Lee, M.G-S., and Van der Ploeg, L. 1991. Gene 105, 255.
          Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol. 57, 1423.
          MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother. 35, 2125.
          Zhou, J., et al. 1991. Gene 107, 307.
          Bulte, L., and Bennoun, P. 1990. Current Genetics 18, 155.
          Giordano, T.J., and McAllister, W.T. 1990. Gene 88, 285.
          Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 24, 1915.
          Carrasco, L., et al. 1989. Pharmacol. Ther. 9, 311.
          Crespi, C.L., et al. 1989. Carcinogenesis 10, 295.
          Damm, B., et al. 1989. Mol. Gen. Genetics 217, 6.
          Egelhoff, T.T., et al. 1989. Mol. Cell. Biol. 9, 1965.
          Kronstad, J.W., et al. 1989. Gene 79, 97.
          Perlin, D.S., et al. 1988. J. Biol. Chem. 263, 118.
          Cullen, D., et al. 1987. Gene 57, 21.
          Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.
          Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun. 117, 6.
          Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.
          Product Information
          CAS number31282-04-9
          FormAmber to brown liquid
          Hill FormulaC₂₀H₃₇N₃O₁₃
          Chemical formulaC₂₀H₃₇N₃O₁₃
          Structure formula ImageStructure formula Image
          Quality LevelMQ100
          Biological Information
          Biological activityThe activity of Hygromycin B is measured in a zonal inhibition assay using Bacillus subtilis. The activity is measured relative to a reference standard.
          Primary Targetgrowth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells
          Secondary targetprotein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA
          Purity>85% by HPLC
          Safety Information according to GHS
          RTECSWK2130000
          Safety Information
          R PhraseR: 26/27/28-37/38-41

          Very toxic by inhalation, in contact with skin and if swallowed.
          Irritating to respiratory system and skin.
          Risk of serious damage to eyes.
          S PhraseS: 23-26-36/37/39-45

          Do not breathe fumes.
          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing, gloves and eye/face protection.
          In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Highly Toxic & Carcinogenic / Teratogenic
          Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsAqueous stock solutions should be kept at concentrations of ≥50 mg/ml and can be stored at either 4°C or -20°C. Aqueous stock solutions (≥50 mg/ml) are stable for up to 6 months at 4°C or -20°C. Working solutions (< 2 mg/ml) are stable for up to 1 month at 4°C.

          Note: This product is highly toxic. Read enclosed MSDS before handling.
          Global Trade Item Number
          Catalogue Number GTIN
          400051-1MU 04055977212174
          400051-5MU 07790788049966