Millipore Sigma Vibrant Logo

100327 Hydrochloric acid in ethanol

for microscopy

Hydrochloric acid in ethanol MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Hydrogen chloride solution

View Products on Sigmaaldrich.com
100327
View Pricing & Availability

Overview

Replacement Information

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
1003275000
Retrieving availability...
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      		 Plastic container 5 l
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      —
      Request Pricing
      		 —
      Description
      Catalogue Number100327
      SynonymsHydrogen chloride solution
      DescriptionHydrochloric acid in ethanol
      OverviewHydrochloric acid in ethanol - for microscopy, is used for human-medical cell diagnosis and serves the purpose of the bacteriological and histological investigation of sample material of human origin. Acid-fast bacteria are difficult to stain because of the high proportion of lipid and wax in their cell walls. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycolatefuchsin complex in the cell wall. With the Kinyoun method, a modification of the AFB-Color carbol fuchsin solution renders heating unnecessary (cold staining). Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol (Cat.no.100327). Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use malachite green is used for counterstaining.
      Pretreatment of the specimens with Sputofluol® dissolves the bacteria from the surrounding viscid sputum and cell material. It is an IVD and CE registered product and can be applied for diagnostical purposes. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
      References
      Product Information
      HS Code3824 99 92
      Quality LevelMQ400
      Applications
      Applicationfor microscopy
      Biological Information
      Physicochemical Information
      Density0.90 g/cm3 (20 °C)
      Flash point21 °C
      pH value1.0 (H₂O, 20 °C)
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Hazard Pictogram(s) Hazard Pictogram(s)
      Hazard Pictogram(s)
      Hazard Statement(s)H225: Highly flammable liquid and vapour.
      H290: May be corrosive to metals.
      H319: Causes serious eye irritation.
      Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
      P233: Keep container tightly closed.
      P234: Keep only in original packaging.
      P240: Ground and bond container and receiving equipment.
      P241: Use explosion-proof electrical/ ventilating/ lighting equipment.
      P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
      P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
      Signal WordDanger
      Storage class3 Flammable liquids
      WGKWGK 1 slightly hazardous to water
      Disposal3
      Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
      Safety Information
      Categories of dangerflammable
      Product Usage Statements
      Storage and Shipping Information
      StorageStore at +2°C to +30°C.
      Packaging Information
      Transport Information
      Declaration (railroad and road) ADR, RIDUN 2924 , 3 (8), II
      Declaration (transport by air) IATA-DGRUN 2924 , 3 (8), II
      Declaration (transport by sea) IMDG-CodeUN 2924 , 3 (8), II, Segregation Group: 1 (Acids)
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      1003275000 04022536004921

      Related Products & Applications

      Accessories (Primary)

      Catalogue Number Description  
      116450 AFB-Color staining kit Show Pricing & Availability

      Accessories (Secondary)

      Catalogue Number Description  
      100497 AFB-Color modified Show Pricing & Availability
      109215 Ziehl-Neelsen carbol-fuchsin solution Show Pricing & Availability

      Related Products By: Application Facete

      Counter Staining
      Bacteria Staining
      Acid-Fast Bacteria Staining

      Categories

      IVD/OEM Materials & Reagents > Microscopy > Universal Reagents > Auxiliaries for Microscopy
      IVD/OEM Materials & Reagents > Microscopy > Bacteriology > Acid-Fast Bacteria Staining