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391338 HEPES, Free Acid, ULTROL® Grade - CAS 7365-45-9 - Calbiochem

A zwitterionic N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C. Absorbance (1.0 M, H2O, 260 nm):≤0.05.

HEPES, Free Acid, ULTROL® Grade - CAS 7365-45-9 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: N-2-Hydroxyethylpiperazine-Nʹ-2-ethanesulfonic Acid

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391338
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
7365-45-9C₈H₁₈N₂O₄S

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                  Description
                  OverviewA zwitterionic N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C.
                  Absorbance (1.0 M, H2O, 260 nm):≤0.05.
                  Absorbance (1.0 M, H2O, 280 nm): ≤0.04
                  Catalogue Number391338
                  Brand Family Calbiochem®
                  SynonymsN-2-Hydroxyethylpiperazine-Nʹ-2-ethanesulfonic Acid
                  References
                  Product Information
                  CAS number7365-45-9
                  FormWhite crystalline powder
                  Hill FormulaC₈H₁₈N₂O₄S
                  Chemical formulaC₈H₁₈N₂O₄S
                  PKA7.25 - 7.75 at 20°C
                  Structure formula ImageStructure formula Image
                  Quality LevelMQ100
                  Applications
                  Biological Information
                  Purity≥99% by titration (dry basis)
                  Physicochemical Information
                  ContaminantsChloride: ≤0.01%; Sodium: ≤0.1%; Sulfate: ≤0.05%
                  Heavy metals≤1 ppm
                  Dimensions
                  Materials Information
                  Toxicological Information
                  Safety Information according to GHS
                  RTECSTL6809000
                  Safety Information
                  R PhraseR: 36/37/38

                  Irritating to eyes, respiratory system and skin.
                  S PhraseS: 26-37/39

                  In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
                  Wear suitable gloves and eye/face protection.
                  Product Usage Statements
                  Storage and Shipping Information
                  Ship Code Ambient Temperature Only
                  Toxicity Irritant
                  Storage +15°C to +30°C
                  Do not freeze Ok to freeze
                  Special InstructionsFollowing reconstitution, store at room temperature (20°C). Stock solutions are stable for up to 6 months at 20°C.
                  Packaging Information
                  Transport Information
                  Supplemental Information
                  Specifications
                  Global Trade Item Number
                  Catalogue Number GTIN
                  391338-100GM 07790788049805
                  391338-1KG 07790788049812
                  391338-25GM 04055977190168
                  391338-500GM 07790788049829

                  Documentation

                  HEPES, Free Acid, ULTROL® Grade - CAS 7365-45-9 - Calbiochem SDS

                  Title

                  Safety Data Sheet (SDS) 

                  HEPES, Free Acid, ULTROL® Grade - CAS 7365-45-9 - Calbiochem Certificates of Analysis

                  TitleLot Number
                  391338

                  Brochure

                  Title
                  Bulk Product Guide
                  Data Sheet

                  Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

                  Revision26-October-2009 RFH
                  SynonymsN-2-Hydroxyethylpiperazine-Nʹ-2-ethanesulfonic Acid
                  DescriptionA zwitterionic N-substituted aminosulfonic acid buffer. HEPES has its maximum buffering capacity very near the pH of mammalian tissue fluids (pKa of 7.31 at 37°C). Exhibits no metal binding and has a small temperature dependent ΔpKa of -0.014/°C.
                  FormWhite crystalline powder
                  CAS number7365-45-9
                  RTECSTL6809000
                  Chemical formulaC₈H₁₈N₂O₄S
                  Structure formulaStructure formula
                  Purity≥99% by titration (dry basis)
                  ContaminantsChloride: ≤0.01%; Sodium: ≤0.1%; Sulfate: ≤0.05%
                  SolubilityH₂O (1 M)
                  Storage +15°C to +30°C
                  Do Not Freeze Ok to freeze
                  Special InstructionsFollowing reconstitution, store at room temperature (20°C). Stock solutions are stable for up to 6 months at 20°C.
                  Toxicity Irritant
                  Merck USA index14, 4654

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                  Categories

                  Life Science Research > Inhibitors and Biochemicals > Biochemicals > Buffers