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109204 Giemsa's azur eosin methylene blue solution

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109204
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1092040100
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      Glass bottle 100 ml
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      1092040500
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          Plastic bottle 500 ml
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          1092041000
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              Plastic bottle 1 l
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              1092041022
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                  Plastic bottle 20 PC
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                  1092042500
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                      Plastic bottle 2.5 l
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                      1092049025
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                          Barrel PE/met 25 l
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                          PE11092041022/20
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                              1 PC
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                              Description
                              Catalogue Number109204
                              DescriptionGiemsa's azur-eosin-methylene blue solution
                              OverviewGiemsa’s Azure Eosin Methylene Blue solution - for microscopy, can be used for the staining of blood and bone marrow smears, paraffin sections and clinical-cytological specimens. Giemsa staining is one of the standard procedures in histology, demonstrating various cells with their morphological features better than with H&E stain. When used in hematological applications, Giemsa’s stain is frequently used in combination with other staining solutions, e. g. with May-Grünwald’s solution for Pappenheim (MGG) overview staining. This ready-to-use staining solution generally stains the nuclei red, based on the molecular interaction between the eosin Y dye and an Azure B-DNA complex. Both dyes assemble to an Eosin Y - Azure B-DNA complex and the intensity of the resulting stain depends on the content of Azure B and the ratio of Azure B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. In histology and clinico-cytological applications, Giemsa’s staining without additional stainings is used as an extended overview staining method. In this method, the color of the various cell components is influenced by pretreatment of the specimen material. Here, chromatin-containing structures (e. g. cell nuclei) appear in various blue shades, while the acidophilic components show up in a variety of red shades. The 500 ml bottle is sufficient for 3500-5000 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
                              References
                              Product Information
                              HS Code3212 90 90
                              Quality LevelMQ400
                              Applications
                              Applicationfor microscopy
                              Biological Information
                              Physicochemical Information
                              Boiling point>65 °C (1013 hPa)
                              Density0.99 g/cm3 (20 °C)
                              Explosion limit5.5 - 44 %(V) Methanol
                              Flash point18 °C
                              Ignition temperature455 °C
                              pH value6.0 - 8.2 (H₂O, 20 °C) (undiluted)
                              Dimensions
                              Materials Information
                              Toxicological Information
                              Safety Information according to GHS
                              Hazard Pictogram(s) Hazard Pictogram(s)
                              Hazard Pictogram(s)
                              Hazard Pictogram(s)
                              Hazard Statement(s)H225: Highly flammable liquid and vapour.
                              H301 + H311 + H331: Toxic if swallowed, in contact with skin or if inhaled.
                              H317: May cause an allergic skin reaction.
                              H370: Causes damage to organs.
                              Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
                              P233: Keep container tightly closed.
                              P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
                              P301 + P310: IF SWALLOWED: Immediately call a POISON CENTER/doctor.
                              P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
                              P304 + P340 + P311: IF INHALED: Remove person to fresh air and keep comfortable for breathing. Call a POISON CENTER/doctor.
                              P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
                              Signal WordDanger
                              Storage class3 Flammable liquids
                              WGKWGK 2 obviously hazardous to water
                              Disposal9
                              Carcinogenic compounds and flammable compounds labelled "Highly toxic" or "Toxic": container F. Alkyl sulfates are carcinogenic; Take particular care to avoid inhalation and skin contact. To neutralize alkyl sulfates, add dropwise (from a dropping funnel) to concentrated ice-cool Ammonia solution (Cat.No. 105426) with vigorous stirring. Before placing in container D, check the pH with pH Universal indicator strips (Cat.No. 109535).
                              Safety Information
                              Categories of dangerhighly flammable, toxic
                              Product Usage Statements
                              Storage and Shipping Information
                              StorageStore at +15°C to +25°C.
                              Packaging Information
                              Transport Information
                              Declaration (railroad and road) ADR, RIDUN 1230 , 3 (6.1), II
                              Declaration (transport by air) IATA-DGRUN 1230 , 3 (6.1), II
                              Declaration (transport by sea) IMDG-CodeUN 1230 , 3 (6.1), II
                              Supplemental Information
                              Specifications
                              Suitability for microscopypasses test
                              Erythrocytesreddish-brownish
                              Nuclei / Chromatinred to violet
                              Eosinophilic granulesreddish to red-brown
                              Neutrophilic granuleslight violet
                              Lymphocyte cytoplasmnblue
                              Monocyte cytoplasmgrey-blue
                              Thrombocytesviolet
                              Basophilic granulesdark violet
                              Nuclei (Tissue section)blue to dark blue
                              Cellsblue to dark blue
                              Collagenpale blue
                              Osteoidpale blue
                              Eosinophilic granula (Tissue section)red
                              Acidic mucopolysaccharidesreddish-violet
                              Mast cell granulesreddish-violet
                              Cartilage matrixreddish-violet
                              Acidophilic materialsorange-red
                              Helicobacter pylori
                              blue to dark blue
                              blue to dark blue
                              Global Trade Item Number
                              Catalogue Number GTIN
                              1092040100 04022536111841
                              1092040500 04022536111889
                              1092041000 04022536720081
                              1092041022 04022536707389
                              1092042500 04022536111926
                              1092049025 04022536111940
                              PE11092041022/20 04061839350878

                              Documentation

                              Giemsa's azur eosin methylene blue solution SDS

                              Title

                              Safety Data Sheet (SDS) 

                              Giemsa's azur eosin methylene blue solution Certificates of Analysis

                              Product Number PackagingSpecificationLot Number
                              1092040100 Glass bottlePDF Specification Document
                              1092040500 Plastic bottlePDF Specification Document
                              1092041000 Plastic bottlePDF Specification Document
                              1092041022 Plastic bottlePDF Specification Document
                              1092042500 Plastic bottlePDF Specification Document
                              1092049025 Barrel PE/metPDF Specification Document
                              PE11092041022/20 PDF Specification Document

                              User Guides

                              Title
                              User Guide (IFU) - 109204

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                              Categories

                              IVD/OEM Materials & Reagents > Microscopy > Hematology > Rapid Stainings, Hemacolor®
                              IVD/OEM Materials & Reagents > Microscopy > Histology > Histological Staining Solutions
                              IVD/OEM Materials & Reagents > Microscopy > Cytology > Cytological Staining Reagents
                              IVD/OEM Materials & Reagents > Microscopy > Hematology > Hematological Staining Solutions
                              IVD/OEM Materials & Reagents > Microscopy > Bacteriology > General Bacteriological Staining Solutions