CellASIC® ONIX Microfluidic Plates |
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The CellASIC® ONIX2 Microfluidic Plates deliver unprecedented control for live cell analysis. Our innovative, easy-to-use format is redefining the standard for microfluidics-based experimentation.
Pre-primed, disposable plates - When you receive the plates, they are "ready to go." Pipette in your cells and sample solutions and begin your experiment.
CellASIC® ONIX2 Microfluidic Plates Key Features:
- Wide range of plate designs for many cell and experiment types
- Innovative, bio-inspired cell culture chambers
- Dynamic control over flow, gas and temperature
- Laminar flow for rapid solutions switching and stable gradient formation
- Perfusion barriers allow continuous mass transport without shear stress
- Multiple experiments can be run in parallel
- Four independent chambers under a single analysis window
- #1.5 (170 µm) thick glass bottom for high quality analysis
- Integrated gas diffusion channels
- 96-well plate footprint
- Compatible with any standard inverted microscope
Using the Plates
| 1. Load cells.
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| 2. Load media or reagents.
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| 3. Seal plate to manifold.
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| 4. Place assembly on microscope.
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| 5. Create protocol using software.
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| 6. Run protocol, perform live cell analysis.
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Microfluidic Plates for Mammalian Cells
The M-series microfluidic plates enable high quality, long term live cell analysis with solution switching. The cell culture chambers are designed to support numerous cell types and applications.
Features and Benefits:
- Advanced microfluidic chamber design promotes cell health
- Laminar flow switching between 5 inlet solutions ensures minimal shear stress on cells
- Perform live-cell analysis experiments for 3+ days on the microscope stage
The M04 plates enable continuous perfusion culture for long-term, live cell analysis. Continuous perfusion of medium and steady removal of waste ensures a suitable growth environment for long-term experiments. Typical experiments run for 4-72 hours on the microscope stage, enabling single cell tracking during exposure, washout, and switching. Cells are easily loaded via pipet, and can be cultured for many days prior to experiment using gravity-driven flow in any incubator.
NIH 3T3 fibroblasts cultured in the M04S Microfluidic Plate and imaged over three days.
Microfluidic Plates for Bacterial Cells
The B-Series microfluidic plates keep bacteria cells in a single focal plane, allowing you to follow and induce cell events during high magnification analysis over many generations.
Features and Benefits:
- Elastic trap sites holds cells in single focal plane for 16+ hours
- Laminar flow switching between 5 inlet solutions
The B04 plates utilize a microfabricated silicone ceiling with a height similar to bacteria cells to restrict their growth in a single focal plane and maintain x,y position over time. Each chamber contains 6 tapered trap heights to maximize loading efficiency of various cell sizes. The B04A plate has heights of 0.7, 0.9, 1.1, 2.0, 3.0 and 4.0 µm.
Continuous perfusion of culture medium to the cells ensures a suitable growth environment for long term experiments. Typical experiments run for 4-16 hours on the microscope stage, enabling single cell tracking for over 10 doublings. The six inlets to each chamber allow solution switching experiments during time lapsed analysis. The highly laminar flow results in a rapid and complete solution change in under a minute.
Growth of E coli (BL21) in the B04 microfluidic plate. Cells were perfused with LB medium and imaged on a 100X oil immersion phase contrast objective. Cells courtesy of Tim Lu, MIT.
Microfluidic Plates for Yeast Cells
The Y-Series microfluidic plates keep yeast cells in a single focal plane, allowing you to follow and induce cell events during high magnification analysis over many generations.
Features and Benefits:- Elastic trap sites holds cells in single focal plane for 16+ hours
- Laminar flow switching between 6 inlet solutions
The Y04 plates utilize a microfabricated silicone ceiling with a height similar to yeast cells to restrict their growth in a single focal plane and maintaining x,y position over time. Each chamber contains 3 tapered trap heights to maximize loading efficiency of various cell sizes. The Y04C plate has heights of 3.5, 4.5 and 5.0 µm, and the Y04D plate has heights of 5, 6, 7 µm. (See Product Sheet for more detail.)
Continuous perfusion of culture medium to the cells ensures a suitable growth environment for long term experiments. Typical experiments run for 4-16 hours on the microscope stage, enabling single cell tracking for over 10 doublings. The six inlets to each chamber allow solution switching experiments during time lapsed analysis. The highly laminar flow results in a rapid and complete solution change in under a minute.
Cell division of S. cerevisiae over 15 hours with continuous flow in the Y04 microfluidic plate. Note the uniformity of focal plane during the entire period. Views taken with a 40x phase objective.