70905 Sigma-AldrichpTriEx™-4 Ek/LIC Vector Kit - Novagen
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Overview
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| Overview | The pTriEx™-4 Ek/LIC vector is an Ek/LIC version of pTriEx-4. The pTriEx-4 Ek/LIC vector is uniquely designed to allow rapid characterization of target genes in multiple expression systems. With this vector, a single recombinant plasmid can be used to test expression in E. coli, insect and mammalian cells. Transient mammalian expression is mediated by the CMV immediate early enhancer and promoter. For expression in insect cells, the pTriEx-4 Ek/LIC vector contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector™ System or BacMagic™ Kits. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. The pTriEx-4 Ek/LIC vector contains N-terminal His•Tag® and S•Tag™ coding sequences followed by an enterokinase protease cleavage site and a multiple cloning site, with the option to include a C-terminal His•Tag coding sequence. |
This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restriction to licensing@milliporesigma.com. This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form. |
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| Catalogue Number | 70905 |
| Brand Family | Novagen® |
| Synonyms | InsectDirect™, pTriEx™-4 Ek/LIC Vector Kit |
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| Components | |
| Quality Level | MQ100 |
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| Ship Code | Dry Ice Only |
| Toxicity | Multiple Toxicity Values, refer to MSDS |
| Storage | ≤ -70°C |
| Do not freeze | Ok to freeze |
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| Specifications |
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| Global Trade Item Number | |
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| Catalogue Number | GTIN |
| 70905 | 0 |
Documentation
pTriEx™-4 Ek/LIC Vector Kit - Novagen Certificates of Analysis
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Brochure
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| Insect Cell Expression |
| Radiance! Multisystem Protein Expression |
User Protocols
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| TB053 Academic and Non-profit Laboratory Assurance Letter |
| TB163 Ek/LIC Cloning Kits |
| TB250 pTriEx™ System Manual |
Vector Map
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| TB419VM pTriEx-4 Ek/LIC Vector |
Vector Sequence
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| pTriEx™-4 Ek/LIC Sequence |