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71229 pSTBlue-1 Perfectly Blunt® Giga Cloning Kit - Novagen

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71229
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Overview

Products

Catalogue NumberPackaging Qty/Pack
71229-3 Plastic ampoule 20 rxn
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Description
OverviewCloning kit with higher-efficiency competent cells

The pSTBlue-1 Perfectly Blunt® Giga Cloning Kits feature NovaBlue GigaSingles™ Competent Cells for increased transformation efficiency (> 1 × 109 cfu/µg). NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue-white screening capability (with appropriate plasmids) and recA endA mutations, which facilitate high yields of excellent-quality plasmid DNA. Cells are provided in a 50-µl, single-use volume that eliminates the need to dispense, freeze/thaw or waste partially used vials.

The Perfectly Blunt Cloning Kits are designed for simplified cloning of DNA generated by PCR using any type of DNA polymerase. This approach enables the use of high-fidelity proofreading enzymes for amplification, thus decreasing the probability of generating mutations in the target sequence. In addition, under many conditions blunt cloning is more efficient than T-cloning, most likely due to the observation that the efficiency of single dA addition by Taq DNA polymerase varies significantly depending on the sequence context of the DNA ends, and even the number of PCR cycles performed (1–5).

Note that the Perfectly Blunt method is not limited to cloning PCR products; these kits are also suitable for cloning restriction fragments, cDNA, or sheared DNA with the same protocols.

The pSTBlue-1 vector is available in a kit containing sufficient reagents for 20 or 40 reactions. "Vector only" kits are also available in 20 and 40 reaction sizes without ligase and competent cells.
Catalogue Number71229
Brand Family Novagen®
Features and benefits
  • No restriction enzymes or special primers
  • Compatible with any DNA polymerase
  • Independent of 3ʹ-dA addition or sequence context of DNA ends
  • Blue/white screening with seven different vectors, including pETBlue expression vectors
  • Simple protocol takes less than one hour* from PCR product to plating transformants

* All times listed refer to kits using NovaBlue Singles™ Competent Cells and ampicillin or chloramphenicol selection. Giga kits, which use NovaBlue GigaSingles™ Competent Cells, require one hour for outgrowth.
References
References1. Novy, R. E., Yaeger, K. W., and Kolb, K. M. (1996) inNovations 6 7–11.

2. Clark, J. M. (1988) Nucleic Acids Res. 16 9677–9686.

3. Brownstein, J. M., Carpten, J. D., and Smith, J. R. (1996) BioTechniques 20 1004–1010.

4. Magnuson, V. L., Ally, P. S., Nylund, S. J., Karanjuwala, Z. E., Rayman, J. B., Knapp, J. I., Lowe, A. L., Ghosh, S., and Collins, F. S. (1996) BioTechniques 21 700–709.

5. Hu, G. (1993) DNA and Cell Biology 12 763–770.
Product Information
Components
Quality LevelMQ100
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage ≤ -70°C
Do not freeze Ok to freeze
Global Trade Item Number
Catalogue Number GTIN
71229-3 04055977271157