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605204 Thrombin, Immobilized, Human Plasma

Thrombin, Immobilized, Human Plasma MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Overview

Replacement Information
Description
OverviewNative thrombin from human plasma, bound to agarose beads.
Catalogue Number605204
Brand Family Calbiochem®
References
ReferencesXiang, B., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 7412.
Product Information
FormSuspension
FormulationIn 100 mM NaCl, 20 mM Tris-HCl, ≤0.1% sodium azide, pH 7.4.
Quality LevelMQ100
Applications
Biological Information
SourcePrepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
605204 0

Documentation

Thrombin, Immobilized, Human Plasma SDS

Title

Safety Data Sheet (SDS) 

Thrombin, Immobilized, Human Plasma Certificates of Analysis

TitleLot Number
605204

References

Reference overview
Xiang, B., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 7412.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-May-2008 RFH
DescriptionNative thrombin from human plasma, bound to agarose beads. Thrombin is a key enzyme in the coagulation cascade. Converts fibrinogen to fibrin and activates factor XIII, which cross-links and stabilizes the fibrin polymer.
FormSuspension
FormulationIn 100 mM NaCl, 20 mM Tris-HCl, ≤0.1% sodium azide, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Recommended reaction conditions
Guidelines for Fusion Protein Cleavage: Adapted from Xiang, et al (see references) Please note that this protocol is provided only as a general guide. Researchers should standardize this assay for their own specific needs and should consult published literature. 1. Immobilized thrombin can be used either with a column or in batch format. For use with batch method the gel may be centrifuged at 3000 rpm or less for separation of agarose from supernatant containing cleaved protein. Higher speeds will result in difficulty resuspending the agarose. 2. A rule of thumb wt/wt ratio to obtain efficient cleavage for most fusion proteins is 1:10 to 1:100 (thrombin:fusion protein) 3. Determine the amount of thrombin required to achieve desired cleavage. Add buffer to this amount of immobilized thrombin to form a slurry. Acceptable buffers include Tris-HCl, containing 150 mM NaCl, citrate buffer, or PBS; the optimal pH is 7.4. For column cleavage, wash the column 2–3 times with buffer prior to addition of sample. The protein should also be resuspended in the same buffer prior to cleavage. 4. Incubation: 25°C, generally overnight; time course including 2 h, 5 h, and 18 h is recommended; assess cleavage by SDS-PAGE. It may be necessary to check cleavage at intermittent intervals (e.g. every 30 min.). 5. For column cleavage, protein that migrates through the column may be monitored at 280 nm.
SourcePrepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
Merck USA index14 9383
ReferencesXiang, B., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 7412.