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58656 Thermolysin, Geobacillus stearothermophilus

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Overview

Replacement Information
Description
Overview

This product has been discontinued.



Native thermolysin from Geobacillus stearothermophilus. Zinc metalloendoproteinase with specificity toward isoleucine, leucine, methionine and valine. Inhibitors include EDTA, 1,10-phenanthroline, phosphinates, and phosphoamidates. Not inhibited by aprotinin or other serine protease inhibitors. Contains a zinc atom essential for activity and four Ca2+ essential for thermal and conformational stability. Note: 1 KU = 1000 units.
Note: 1 KU = 1000 units.
Catalogue Number58656
Brand Family Calbiochem®
References
ReferencesPfuetzner, R.A. and Chan, W.W. 1993. Eur. J. Biochem. 218, 523.
Matsubara, H. 1970. Methods Enzymol. 19, 642.
Ohta, Y., et al. 1966. J. Biol. Chem. 241, 5919.
Product Information
CAS number37246-64-3
Activity≥7,000,000 PU/g dry weight

PU = protease unit
Unit of DefinitionOne protease unit is defined as the amount of the enzyme that liberates the non-proteinous digestion product from milk casein at final concentration of 0.5% to give Folin's color equivalent to 1 µg of tyrosine per min at the early stage of the reaction at 35°C, pH 7.2.
EC number3.4.24.2
FormLyophilized
FormulationLyophilized solid containing calcium acetate, sodium acetate, 3X crystallized.
Hygroscopic Hygroscopic
Quality LevelMQ100
Applications
Biological Information
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSXH7500000
Safety Information
R PhraseR: 20-36/37/38-42/43

Harmful by inhalation.
Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation and skin contact.
S PhraseS: 22-26-36

Do not breathe dust.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage +2°C to +8°C
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
58656 0

Documentation

Thermolysin, Geobacillus stearothermophilus SDS

Title

Safety Data Sheet (SDS) 

Thermolysin, Geobacillus stearothermophilus Certificates of Analysis

TitleLot Number
58656

References

Reference overview
Pfuetzner, R.A. and Chan, W.W. 1993. Eur. J. Biochem. 218, 523.
Matsubara, H. 1970. Methods Enzymol. 19, 642.
Ohta, Y., et al. 1966. J. Biol. Chem. 241, 5919.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-June-2015 JSW
DescriptionNative thermolysin from Geobacillus stearothermophilus. A highly thermostable zinc metallo-endoproteinase that exhibits specificity toward isoleucine, leucine, methionine, and valine. Inhibitors include EDTA, 1,10-phenathroline, phosphinates, and phosphoamidates. Not inhibited by aprotinin or other serine protease inhibitors. Contains a zinc atom essential for activity and four Ca2+ essential for thermal and conformational stability. Has an optimal pH in the range 7.0-9.0.
FormLyophilized
FormulationLyophilized solid containing calcium acetate, sodium acetate, 3X crystallized.
Concentration Label Please refer to vial label for lot-specific concentration
CAS number37246-64-3
RTECSXH7500000
EC number3.4.24.2
Activity≥7,000,000 PU/g dry weight

PU = protease unit
Unit definitionOne protease unit is defined as the amount of the enzyme that liberates the non-proteinous digestion product from milk casein at final concentration of 0.5% to give Folin's color equivalent to 1 µg of tyrosine per min at the early stage of the reaction at 35°C, pH 7.2.
SolubilityReconstitute in 42% glycerol adjusted to pH 8.0 with 0.01 N NaOH, containing 0.005% Triton® X-100 detergent.
Storage +2°C to +8°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Toxicity Harmful
Merck USA index14, 9286
ReferencesPfuetzner, R.A. and Chan, W.W. 1993. Eur. J. Biochem. 218, 523.
Matsubara, H. 1970. Methods Enzymol. 19, 642.
Ohta, Y., et al. 1966. J. Biol. Chem. 241, 5919.

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Categories

Life Science Research > Proteins and Enzymes > Other Enzymes