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654245 TNF-α, Mouse, Recombinant, E. coli

TNF-α, Mouse, Recombinant, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: rmTNF-α, rm-Cachectin, Tumor Necrosis Factor-α

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654245
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
654245-10UG Plastic ampoule 10 μg
Description
OverviewRecombinant, mouse tumor necrosis factor-α expressed in E. coli. Has the ability to kill certain tumor cells directly. Referred to as an inflammatory cytokine as it initiates the cascade of other cytokines and factors that make up the immune systems response to infection and cancer. Reported to mediate changes in bone metabolism during inflammation.
Catalogue Number654245
Brand Family Calbiochem®
SynonymsrmTNF-α, rm-Cachectin, Tumor Necrosis Factor-α
References
ReferencesScharla, S.H., et al. 1994. Eur. J. Endocrinol. 131, 293.
Eck, M.J., et al. 1992. J. Biol. Chem. 267, 2119.
Product Information
FormLyophilized
FormulationLyophilized from sterile-filtered PBS, 50 µg BSA/µg TNF-α.
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀ = 20-50 pg/ml as measured in a cytotoxic assay using the TNF-α susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)
Purity≥97% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤100 pg/µg TNF-α
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
654245-10UG 04055977184600

Documentation

TNF-α, Mouse, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

TNF-α, Mouse, Recombinant, E. coli Certificates of Analysis

TitleLot Number
654245

References

Reference overview
Scharla, S.H., et al. 1994. Eur. J. Endocrinol. 131, 293.
Eck, M.J., et al. 1992. J. Biol. Chem. 267, 2119.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-May-2008 RFH
SynonymsrmTNF-α, rm-Cachectin, Tumor Necrosis Factor-α
DescriptionRecombinant, mouse tumor necrosis factor-α expressed in E. coli. Has the ability to kill certain tumor cells directly. It is referred to as an inflammatory cytokine as it initiates the cascade of other cytokines and factors that make up the immune system's response to infection and cancer. Reported to mediate changes in bone metabolism during inflammation.
FormLyophilized
FormulationLyophilized from sterile-filtered PBS, 50 µg BSA/µg TNF-α.
Purity≥97% by SDS-PAGE
ContaminantsEndotoxin: ≤100 pg/µg TNF-α
Biological activityED₅₀ = 20-50 pg/ml as measured in a cytotoxic assay using the TNF-α susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)
SolubilityReconstitute to a concentration ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
Toxicity Standard Handling
Merck USA index14, 9812
ReferencesScharla, S.H., et al. 1994. Eur. J. Endocrinol. 131, 293.
Eck, M.J., et al. 1992. J. Biol. Chem. 267, 2119.